内蒙古东部地区奶牛布鲁氏菌病流行学调查及分离与鉴定

Epidemiological Survey,Separation and Identification of Cow Brucellosis in Eastern Inner Mongolia

为掌握内蒙古自治区东部地区奶牛布鲁氏菌病的流行现状和流行特征,在试验中采用全乳环状试验(milk ring test,简称MRT)、奶液间接酶联免疫吸附试验(indire enzyme-linked immunosorbent assay,简称iELISA)两种方法对科尔沁地区5个牛场进行了布病流行学调查,比较两种方法对检测布鲁氏菌病阳性牛的差异性。通过对内蒙古自治区东部地区5个盟市进行牛布鲁氏菌病流行学调查;最后对检出的部分阳性奶牛乳汁进行细菌分离培养,用VirB8和AMOS方法进行扩增鉴定。表明两种方法(MRT和iELISA)检测结果差异不显著;经检测17株分离株均为布鲁氏菌。布鲁氏菌分子分型扩增的鉴定显示,牛场感染的菌株为牛种布鲁氏菌。内蒙古东部地区部分饲养场布鲁氏菌病阳性率为1.67%。

In order to investigate the epidemic situation and epidemiological characteristics of the brucellosis from the cows in Eastern Inner Mongolia, two methods （Milk Ring Test, MRT; Milk Indire Enzyme-linked Immunosorbent Assay, iELISA） were used to do epidemiological survey in the five cattle farms of KeErQin area, and the difference of these two methods on the detection of brucellosis positive cow were compared. Survey of brucellosis epidemiology in eastern Inner Mongolia was conducted, from which the bacteria of partial positive milk was isolated and cultured, and VirB8 and AMOS methods were adopted to amplify and identify it. It was suggested that the differences of the results measured by the two methods （MRT and iELISA） were not significant; After detection, all the 17 isolated strains were Brucella. The results of the identification of Brucella molecular typing amplification showed that it was the Brucella that infected cattle farms. The positive rate of Brucella in eastern Inner Mongolia farms was 1.67%.