硅酸盐细菌LJP13的分离鉴定及生长特性

Isolation Identification and Growth Characteristics of Silicate Bacterium LJP13

从‘凤丹’牡丹根际土壤中分离到一株硅酸盐细菌,编号为LJP13。在28℃、180 r/min条件下发酵培养7 d后,发酵液中水溶性速效钾含量达到1.3μg/mL,从而确定LJP13具有解钾活性,并定性为硅酸盐菌株。通过形态特征观察、生化特性测定和16S rDNA聚类分析,初步将菌株LJP13鉴定为芽孢杆菌属Bacillus菌株,暂命名为Bacillus sp.LJP13。对菌株LJP13的生长特性研究表明,菌株对几种底物的发酵产气能力顺序是麦芽糖>葡萄糖>直链淀粉>乳糖>甘露醇>支链淀粉;在碳同化利用能力上蔗糖为最适底物。对不同氮源的同化能力顺序为酵母膏>硝酸钾>蛋白胨>硫酸铵>尿素。菌株的最适生长温度为28~32℃,适生pH为7。LJP13菌株可作为牡丹专用菌肥开发的备用菌株。

A strain of silicate bacteria was isolated from rhizosphere soil of Paeonia ostii ＇Fengdan＇, which was coded LJP13. After 7 d fermentation under the condition of 28℃, 180 r/min, the water soluble potassium content in fermentation broth reached 1.3 μg/mL, from which we confirmed that LJP13 had the activity of potassium solution, and it was characterized as silicate strains. Through the observation of its morphology, detection of biochemical characteristics and 16S rDNA clustering analysis, LJP13 was identified as Bacillus sp.,and temporarily named as Bacillus sp. LJPI3. The analysis of the growth characteristics of strain LJP13 showed that the ability of the strain to produce fermentation of several substrates was maltose 〉glucose 〉straight chain starch 〉lactose 〉 mannitol〉amylopectin; Sucrose was the optimum substrate in carbon assimilation and utilization. The order of assimilation of different nitrogen sources was yeast extract〉potassium nitrate〉peptone〉ammonium sulfate〉urea. The optimum growth temperature for strain was 28-32℃, and the optimum pH was 7. LJP13 could be used as an alternate strain for the development of special fertilizer of Peony.