藏族和汉族健康人群血浆miRNA表达谱的差异

Difference of miRNA expression profile in plasma between Tibetan and Han nationality

目的检测并比较藏族和汉族健康人群血浆miRNA表达谱。方法分别采集246例藏族健康人和128例汉族健康人血浆样本,用Taq Man低密度芯片技术对50例藏族和50例汉族组成的2组人混合血浆中的754种miRNA进行检测,随机选取存在miR-130a-3p和miR-629-5p表达差异的样本,用qRT-PCR法进行验证。结果低密度芯片结果显示,藏族和汉族健康人群血浆中miRNA表达谱的相关系数(r)为0.592。与汉族组相比,藏族组血浆中139种miRNA的表达水平发生明显改变,其中62种miRNA表达上调,77种miRNA表达下调。qRT-PCR进一步证实miR-130a-3p和miR-629-5p在藏族组血浆中的含量明显高于汉族组[(467±27.30)×10-5vs(236±9.69)×10-5和(14.67±0.94)×10-5vs(7.58±0.52)×10-5,P均<0.01]。结论藏族人群血浆miRNA的表达谱与汉族人群存在明显差异,临床检测miRNA时应考虑民族因素的影响。

Objective To measure and compare the expression profiles of plasma miRNA between Tibetan and Han nationality. Methods Plasma samples were taken from 246 healthy Tibetans and 128 Han individuals. The randomly selected 50 Tibetan and Hart plasma samples were pooled respectively and the levels of 754 miRNAs were examined using a TaqMan Low Density Array. Two markedly differentially expressed miRNAs,miR-130a-3p and miR-629-Sp,were verified in all the plasma samples by individual qRT-PCR. Re- suits The Low Density Array results showed that the correlation coefficient of expression profiles of plasma miRNA for the Tibetan and Han population was 0.592. Compared with Han population, the expression levels of 139 miRNAs were distinctly different, in Tibetan （62 up-regulated and 77 down-regulated）. The levels of miR-130a-3p and miR-629-Sp were further verified to be significantly higher in the plasma from Tibetans than those in the plasma from Han population [ （467 ±27.30） × 10^-5 vs（236 ±9.69） × 10^ -5 ,P 〈0.01 ; （14.67±0.94） × 10^-5 vs（7.58 ±0.52） × 10^-5,P〈0.01] by qRT-PCR assay. Conclusion There may be marked difference of plasma miRNA expression profile between Tibetan and Han nationality. The influence of the nationality factors on miRNA profiles should be taken into account in the application of miRNAs in clinical detection in the future.