基于RNA-seq研究胶质母细胞瘤中差异表达基因及蛋白相互作用网络

Study on Differentially Expressed Genes and Protein-Protein Interaction Network in Glioblastoma Based on RNA-seq

本研究基于RNA-seq数据分析了胶质母细胞瘤中的差异表达基因,并对差异表达基因进行了功能(GO term)和通路(KEGG)富集分析。进一步通过蛋白相互作用网络挖掘了胶质母细胞瘤的调控机理。结果表明,405个基因在肿瘤组织中差异表达(p-value≤0.05,|log FC|≥1.5),其中216个(53.3%)基因上调,189个(46.6%)基因下调。基因本体(gene ontology,GO)富集结果表明,这些差异表达基因参与了离子转运,神经冲动传递,细胞信号转导和细胞粘附等。此外,KEGG通路富集结果表明,差异基因参与了许多重要的生物学通路,包括ECM受体相互作用、黏着和钙信号等通路。进一步的蛋白相互作用网络分析鉴定了5个关键的hub基因,包括PTK2B、CDK1、FN1、CCND1和FOS。这5个关键基因对于胶质母细胞瘤的发生和发展可能起到了关键作用,可以作为潜在的调控位点和筛选的标志物。

In this study, the differentially expressed genes （DEGs） in glioblastoma were explored based on RNA-seq data, and the DEGs were further subjected to GO and KEGG enrichment analysis. In addition, the molecular mechanism of glioblastoma was investigated via protein-protein interaction. Results showed that a total of 405 genes were differentially expressed in tumor tissue （p-value ≤ 0.01 and ｜log FC｜〉 1.5）, in which 216 genes （53.3%） were up-regulated and 189 genes （46.6%） were down-regulated. GO function enrichment indicated that the DEGs were involved in ion transport, transmission of nerve impulse, cell-cell signaling and cell adhesion. Also, the result of KEGG nrichment analysis found that the DEGs participated in several important signaling pathways, including ECM （extracellular matrix）-receptor interaction, focal adhesion and calcium signaling. Further protein-protein interaction network analysis demonstrated and identified five hub genes which contained PTK2B （protein tyrosine kinase 2 beta）, CDK1 （cyclin-dependent kinase 1）, FN1 （fibronectin 1）, CCND1 （cyclin D1） and FOS （FBJ murine osteosarcoma）. These five key genes might play an important role in the occurrence and development of glioblastoma, which could be used as potential regulatory sites and screening markers.