摘要
本研究通过比较胃癌与萎缩性胃炎的RUNX3及CHFR基因表达情况探讨其中的相关性。选取2014年1月至2016年7月前来浙江舟山群岛新区旅游与健康职业学院进行胃镜检查的137例慢性萎缩性胃炎患者作为研究对象,分为胃炎轻度组、胃炎中度组与胃炎重度组,并选取同期胃癌患者42例作为胃癌组。取胃炎患者的胃体与胃窦处的黏膜,胃癌患者的癌灶组织、癌旁组织与远端正常处组织进行DNA的提取,并进行比较。经比较后,胃炎重度组患者的RUNX3与CHFR基因甲基化阳性率分别为29.63%、37.04%,与胃癌组患者的正常组织处比较,有显著的统计学差异,与胃癌组癌灶组织处比较无差异;而胃炎重度组患者的蛋白表达有缺失情况发生,经Elisa检测后,蛋白表达量与胃癌组癌灶组织处比较无差异。严重萎缩性胃炎患者的RUNX3与CHFR基因甲基化可抑制抑癌基因的表达,而异常升高的阳性率会影响患者的病程进展,可认为RUNX3与CHFR基因甲基化对癌前病变进程具有临床诊断意义。
This study compared the expression of RUNX3 and CHFR genes in gastric cancer and atrophic gastritis to explore their relationship. 137 cases of chronic atrophic gastritis patients who came to our hospital for gastroscopy from January 2014 to July 2016 were chosen as the research objects, who were divided into mild gastritis group, moderate group and severe gastritis group, and 42 cases of gastric cancer patients were selected into gastric cancer group. The gastric body and gastric antrum mucosa from gastritis patients, and cancer tissue, adjacent tissue and distal normal tissue from gastric cancer patients were collected and extracted the DNA for comparison. After comparision, it was found that positive rate ofmethylation in RUNX3 and CHFR genes in severe gastritis group were 29.63% and 37.04%, respectively, and it was statistically different from the normal tissue of gastric cancer patients, but it had no difference with the tumor tissue from gastric cancer group; However, loss of protein expression occurred in severe gastritis group, but there was no difference in protein expression compared with cancer tissue from cancer group after being detected by Elisa. The methylation of R UNX3 and CHFR gene in severe atrophic gastritis patients could inhibit the expression of tumor suppressor gene, while the abnormal increased positive rate would affect the progression of disease, which could be concluded that the methylation of RUNX3 and CHFR would be of clinical diagnosis significance for precancerous lesion.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第4期1301-1306,共6页
Genomics and Applied Biology
