诊断超声联合微泡对兔VX_2肿瘤的血流增强效应

Vascular effect of rabbit VX_2 tumor induced by diagnostic ultrasound with microbubbles

目的探讨不同机械指数(MI)的诊断超声联合微泡对兔VX_2肿瘤的血流增强效应。方法选取健康雄性新西兰白兔40只,采用单侧大腿内侧瘤组织块接种法接种VX_2肿瘤,造模成功后将其随机分为实验1组(MI=0.3)、实验2组(MI=0.7)、实验3组(MI=1.4)及对照组,每组各10只。抽取0.2 ml"脂氟显"用5.0 ml生理盐水稀释后经耳缘静脉通道匀速推入,同时分别经不同机械指数辐照肿瘤,对照组予以超声假照,时间均为5 min。储存治疗前后动态造影图像,并用造影分析软件分析,记录峰值强度(PI)和曲线下面积(AUC)。结果实验1组、实验3组治疗前后PI比较差异均有统计学意义(P=0.028、0.018),实验2组、对照组治疗前后PI比较差异无统计学意义(P=0.994、0.978);实验3组治疗前后AUC值比较差异有统计学意义(P=0.009),实验1、2组及对照组治疗前后AUC值比较差异均无统计学意义(P=0.099、0.497、0.898)。结论低能量诊断超声(MI=0.3)联合微泡可丰富兔VX_2肿瘤血供,高能量诊断超声(MI=1.4)可减少血流灌注。

Objective To investigate the different vascular effect of rabbit VX2 tumor induced by different mechanical index（MI） of diagnostic ultrasound combined with microbubbles. Methods Forty rabbits with VX2 tumor were treated by diagnostic ultrasound with different MI （0.3,0.7,1.4） with microbubbles as experiment group（experiment group 1, 2,3 ） and negative control without any treatment （control group ）, each group included 10 rabbits. 0.2 ml microbubbles in 5.0 ml normal saline was used as a therapeutic dose, the treatment time was 5 min.The tumor blood perfusion was imaged with contrast-enhanced ultrasound before and after treatment which were analyzed by the contrast analysis software, the peak intensity （PI） and the area under curve （AUC） were obtained. Results There were significant differences of PI before and after the treatment in experiment group 1 and experiment group 3 （P=0.028, 0.018 ）, and PI were not statistically significant between experiment group 2 and control group before and after the treatment（P=0.994, 0.978）. The AUC was significantly different before and after the treatment in the experiment group 3（P=0.009 ）, while there were no statistical significance of AUC before and after the treatment in experiment group 1,2 and control group （P=0.099, 0.497,0.898 ）. Conclusion Low-intensity diagnostic ultrasound （MI=0.3） combined with microbubbles can enrich the blood perfusion of VX2 tumor in rabbits, and high-intensity diagnostic ultrasound（MI=1.4） can decrease the blood perfusion.