外源NaHS对盐胁迫下板栗幼苗的缓解效应

Alleviation Effect of Exogenous Sodium Hydrosulfide on Chestnut Seedlings under Salt Stress

为探讨外源硫化氢对盐胁迫下板栗幼苗的缓解作用,以两年生红栗2号板栗幼苗为试材,研究不同浓度(0、0.1、0.2、0.5、1.0、2.0 mmol·L^(-1))的硫化氢供体硫氢化钠(NaHS)对100 mmol·L^(-1)NaCl胁迫下板栗幼苗叶片光合特性、叶绿素含量、脯氨酸含量及丙二醛(MDA)含量的影响。结果表明:喷施外源NaHS可不同程度缓解盐胁迫对板栗幼苗叶片造成的伤害,与100 mmol·L-1NaCl胁迫处理相比,叶绿素含量提高,MDA含量降低,脯氨酸含量升高,从而有效降低了盐胁迫下细胞膜受损程度,提高了板栗对盐渍环境的适应性,且这种缓解效应存在明显的剂量效应,以0.5、1.0 mmol·L^(-1)NaHS两个浓度效果较好。

In order to study the alleviation effect of exogenous hydrogen sulfide on chestnut seedlings under salt stress,using two years old chestnut ’Hongli 2’ seedlings as materials,the effects of different concentrations（0,0.1,0.2,0.5,1.0,2.0 mmol·L-1） of sodium hydrosulfide（Na HS） on the photosynthetic characteristics,chlorophyll content,proline content and malondialdehyde（MDA） content of chestnut leaves were investigated under 100 mmol·L-1 NaCl stress.The results showed that spraying exogenous Na HS could alleviate the damage caused by salt stress on the leaves of chestnut seedlings in different degrees.Compared with 100 mmol·L-1 NaCl treatments,the chlorophyll and proline content increased,MDA content significantly dropped,which effectively reduced the damage of cell membrane under salt stress and improved the adaptability of chestnut to saline environment.In addition,the Na HS dosage influenced the alleviation effect obviously,and 0.5 mmol·L-1 and 1.0 mmol·L-1 NaHS had better alleviation effect.