姜黄素联合白藜芦醇抑制人头颈部肿瘤细胞系增殖的机制研究

Curcumin combined with resveratrol enhances their anti-tumoral effects in the head and neck carcinoma

目的探讨姜黄素联合白藜芦醇抑制人头颈部肿瘤细胞系增殖的机制。方法用姜黄素和白藜芦醇联合处理Hep2(人喉癌细胞株)及Fa Du(人下咽癌细胞株),应用MTT(塞唑蓝)比色法检测细胞增殖抑制率,平板克隆增殖实验检测单个细胞集落形成能力,Hoechst33258染色检测细胞凋亡情况,同时应用Real-time PCR法检测Bax和Bcl-2的mRNA表达水平。结果姜黄素和白藜芦醇对Hep2和FaDu细胞增殖有抑制作用,二者联合处理后,对细胞增殖、细胞克隆形成能力及细胞凋亡的抑制均明显增强;同时,姜黄素和白藜芦醇上调细胞中Bax和下调Bcl-2的mRNA水平。结论姜黄素与白藜芦醇联合处理可抑制Hep2及FaDu细胞增殖,作用机制可能与上调Bax、下调Bcl-2基因表达有关。

Objective To study the effect of curcumin in combination of resveratrol on proliferation in the head and neck neoplasm cell lines. Methods Hep2 cells and FaDu cells were treated with Curcumin and resveratrol. MTT was used to determine cell proliferation. Colony formation was used to detect the cell viability. Hoechst 33258 staining was performed to observe the cell apoptosis. The mRNA expressions of Bax and Bcl-2 were analyzed by Real-time PCR.Results Hep2 and FaDu cell proliferation could be inhibited with curcumin and resveratrol in a dose-dependent manner. Their combination exhibited the best inhibitory effects. Curcumin combined with resveratrol exhibited colony repression and showed a more significant apoptosis in Hep2 and Fa Du cells. Real-time PCR demonstrated that Bax mRNA was upregulated and Bcl-2 mRNA was downregulated by curcumin and resveratrol. Meanwhile,Bax mRNA was upregulated and Bcl-2 mRNA was downregulated by curcumin and resveratrol. Conclusion The combination of curcumin and resveratrol can inhibit the proliferation of Hep2 and Fa Du cells more significantly. The potential mechanism may be related with the up-regulation of Bax and the down-regulation of Bcl-2.