HBV相关肝纤维化患者血浆microRNA的差异表达

Differential expression of plasma microRNA in patients with HBV-related liver fibrosis

目的探索microRNA在乙型肝炎肝纤维化患者血浆中的差异表达。方法收集2013年至2014年本院收治的经肝组织活检诊断为肝纤维化S2~S3期的患者10例、代偿期肝硬化患者8例,另选取8例健康志愿者作为对照组,采用IlluminaHiSeq测序技术检测各组血浆miRNA表达,对差异表达的miRNA进行聚类分析和靶基因预测;对差异表达miRNA靶基因进行基因本(GO)分析和KEGG通路富集分析。结果与健康对照组相比,肝纤维化组筛选出104个差异miRNA,其中72个表达上调,32个表达下调;肝硬化组筛选出102个miRNA,其中70个表达上调,32个表达下调。两组中均显著上调的共22个,均显著下调的共24个,在上调的miRNA中,共9个miRNA在肝硬化组表达量高于肝纤维化组;下调的miRNA在肝纤维化组与肝硬化组的表达无显著差异。结论肝纤维化患者血浆miRNA表达与健康组有显著差异,这些差异表达的miRNA在肝纤维化进展中起着重要作用,其可能参与肝纤维化发生发展过程中某些信号通路的调控,确切机制尚需进一步深入研究。

Objective To explore the differential expression of plasma miRNA in patients with HBV-related liver fibrosis. Methods Total of 10 cases with HBV-related liver fibrosis （S2-S3）, 8 cases with compensated liver cirrhosis and 8 healthy controls from 2013 to 2014 in Beijing Ditan Hospital, Capital Medical University were selected. The expression of plasma miRNA were detected by IlluminaHiSeq sequencing. Cluster analysis and target genes prediction of differential expression miRNA were carried out and gene ontology （GO） enrichment analysis and KEGG pathway enrichment analysis were performed for the target genes of differential expression miRNA. Results Compared with healthy group,total of 104 miRNA were screened out from liver fibrosis group, of which 72 miRNA were up-regulated and 32 were down-regulated. In liver cirrhosis group, total of 102 miRNA were screened out, of which 70 were up-regulated and 32 were downregulated.Total of 22 miRNA were up-regulated and 24 miRNA were down-regulated in liver fibrosis group and liver cirrhosis group. Among those up-regulated miRNA, the expression of 9 miRNA in liver cirrhosis group was higher than those in hepatic fibrosis group, while in the down-regulated miRNA, the differences were not significant. Conclusions The expression of miRNA in patients with liver fibrosis is significantly different from those of healthy subjects.miRNA have a vital role in the progess of liver fibrosis. Many signal pathways of liver fibrosis may be regulated by miRNA and the exact mechanism needs further study.