摘要
目的通过对江西省2013年至2015年急性弛缓性麻痹病例病原学监测,为证实江西省消灭脊髓灰质炎提供准确的、可靠的科学依据。方法采用L20B、RD细胞继续脊髓灰质炎病毒分离,脊髓灰质炎毒株通过ITD方法进行型内鉴定,若为复合型毒株再经过血清中和实验分单型后,送至国家脊髓灰质炎实验室进行序列测定。结果 2013年至2015年共接收1058份AFP病例粪便标本,其中有28份分离出脊髓灰质炎病毒,83份分离出非脊髓灰质炎肠道病毒,分离率分别为2.65%,7.84%;接收AFP病例接触者粪便标本57份,分离出脊灰病毒2份和非脊灰肠道病毒7份,平均分离率分别为3.51%,12.28%;在2013年分离出一例疫苗衍生株病例,未发现脊灰野病毒。结论江西省脊髓灰质炎病毒学监测处于较高的水平,江西省2013年至2015年急性弛缓性麻痹病例病中未发现脊灰野病毒,但检出VDPV,提示我们要警惕疫苗株的变异,应继续加强监测。
Abstract：Objective To provide accurate,reliable science evidences for Polio free status in Jiangxi,and to analyze the lab monitoring results of the acute flaccid paralysis （AFP） case from 2013 to 2015. Methods Polio viruses were isolated by L20B cell and RD cell. The isolated Polio strains were typed by ITD test for intratypic identification and then sent to national Polio laboratory for Sequencing. Result We received 1058 AFP stool specimens from 2013 to 2015. The isolates of Polio viruses and NPEV were 27（2.65%） and 83（7.84%）, respectively. Among the 57 received AFP cases contacts stool specimens, 2 were identified as poliovirus and 7 were identified as NPEVs,with a separation rate of 3.51% and 12.28% on average. No wild polio virus was found but VD- PVs were found from cases in 2013. Conclusion Polio virological surveillance in Jiangxi province stay at a high level. No wild polio virus was found in the AFP cases from 2013 to 2015 in Jiangxi province. But the finding of VDPVs warned us we should continue to strengthen surveillance. Key words：
出处
《实验与检验医学》
CAS
2017年第2期171-173,共3页
Experimental and Laboratory Medicine
基金
江西省卫计委课题
编号:20166018
