2015年江西省麻疹疫苗株病毒的快速鉴别

Rapid identification of measles vaccine strain virus in Jiangxi Province in 2015

目的运用逆转录多聚酶链式反应-限制性片段长度多态性分析方法(Reverse Transcription-Polymerase Chain Reaction-Restriction Fragment Length Polymorphism,RT-PCR-RFLP)快速鉴别2015年麻疹疫苗株病毒。方法用RT-PCR-RFLP方法检测两例疑似疫苗相关麻疹病例咽拭子标本是否含有AflⅡ酶切位点鉴别麻疹疫苗株病毒,同时对标本进行N基因羧基末端450个核苷酸进行序列测定分析,在基因层面进一步证实,以验证RT-PCR-RFLP方法。结果 RT-PCR-RFLP方法鉴定的结果为两例疑似疫苗相关麻疹病例咽拭子标本均能被AflⅡ酶切开,N基因序列测定结果为两例疑似疫苗相关麻疹病例咽拭子标本为麻疹疫苗株病毒A基因型。结论首次为江西省麻疹疫苗株病毒提供实验室证据。

Objective Using reverse transcription polymerase chain reaction restriction fragment length polymorphism analysis method to identify rapidly the Measles vaccine strain virus in Jiangxi Province,in 2015. Methods We identifyed two cases of sus- pected vaccine associated measles throat swab specimens by detecting whether they contains All II enzyme cutting site with RT- PCR-RFLP method. At the same time,the carboxyl terminal 450 nucleotides of N gene was sequenced and analyzed,confirming the result obtained by RT-PCR-RFLP method. Results The identification results of RT-PCR-RFLP method is that two throat swab specimens can be All II enzyme incision, and the N gene sequence determination results for measles vaccine strain virus of genotype A. Conclusion Our study provides laboratory evidence for measles vaccine strain in Jiangxi Province for the first time. Key words：