一种制作骨髓染色体G带的改良方法

The improved technique of bone marrow chromosome G-banding

目的建立一种具有分裂相多、分散度好、带纹清晰、长度适中等特征的骨髓染色体G带制作方法。方法在含9.6%胎牛血清的RPMI1640培养基中加入一定量的人淋巴瘤细胞系培养物,接种骨髓细胞后培养24h,然后加入终浓度为30μg/ml的溴化乙锭和0.06μg/ml的秋水仙胺作用1h。结果通过方法改良,骨髓标本培养成功率约为传统方法的1.59倍,异常染色体检出率比传统方法提高了51%。结论改良后的方法可以制作出个数更多、分散度更加良好、带纹更加清晰、染色体更长的中期分裂相,因而异常染色体检出率更高,诊断结果更加可靠。

Objective To build a method for bone marrow chromosome G-banding with more and better metaphase chromosomes. Methods Some culture from human lymphocyte carcinoma cell line was added into the traditional bone marrow medium,then the marrow cells were cultured about 24 h. At the second day,30μg/ml ethidium bromide and 0.06μg/ml colcemid at the final concentration were added into the above medium and incubated about 1h prior to cell harvest. Results The rate of successful chromosome preparation were about 1.59 times the rate of control and the detection rate of chromosome aberrations were improved51%. Conclusion By the improved methods,better metaphase chromosomes could be obtained to be analyzed easily,so the diagnosis would be more exactly.