干旱胁迫对不同品种大豆下胚轴生理生化特性的影响

Effects of drought stress on physiological and biochemical characteristics of soybean hypocotyls

研究分别以2种大豆WDD00172(WDD-172)和晋豆21(JD-21)为实验材料,探讨干旱胁迫对不同品种大豆下胚轴生理特性及抗氧化酶活性的影响。结果表明,随着9%PEG6000胁迫时间的延长,大豆下胚轴MDA含量和离子渗漏显著增高,且WDD-172增幅较大;干旱胁迫下GSH含量、CAT和POD活性持续下降,APX活性则先上升后下降,而且WDD-172下降更加明显。研究发现JD-21更加抗旱可能与体内高浓度的葡萄糖-6-磷酸脱氢酶(G6PDH)活性有关;干旱胁迫下JD-21比WDD-172更加耐受干旱胁迫,可能是由于体内抗氧化酶活性(CAT、POD和APX)和抗氧化物质GSH含量较高的原因。

In this study, WDD00172 （WDD-172） and Jindou 21（JD-21） were used to determine the effects of drought stress on physiological and biochemical characteristics in soybean hypocotyls. These results indi- cated that the content of MDA and electrolyte leakage in soybean hypocotyls were significantly increased in both soybean varieties with an increase of 9% PEG6000 treatment. The content of MDA and electro- lyte leakage showed much higher in WDD- 172 than in JD-21. It also showed that under drought stress, GSH content, CAT and POD activities continued to decline, while the APX activity was increased firstly and then decreased. However, those in WDD-172 decreased more obviously than in JD-21. It was discovered that the activity of glucose -6- phosphate dehydrogenase （G6PDH） was even higher in JD-21 than in WDD-172 in vivo, and JD-21 was more tolerance than WDD-172 under drought stress, which might be due to the higher antioxidant enzyme activities （CAT, POD and APX） and the higher content of antioxidant GSH in the body of JD-21 compared with WDD-172.