水溶性石墨烯转染滑膜间充质干细胞的研究

Transfection Efficiency of Water Soluble Grapheme on Synovial Stem Cells

目的:探究水溶性石墨烯转染滑膜间充质干细胞的的效果及各组间细胞克隆形成率的差别比较。方法:将传代培养到第3代的滑膜间充质干细胞分成实验组与对照组,实验组细胞培养基内加入浓度为25mol/mL的水溶性石墨烯在37℃的条件下培养24h,对照组用普通培养基培养37℃24h,405nm激发波长下的激光共聚胶显微镜下观察石墨烯是否成功转染滑膜干细胞。各组间细胞克隆形成率的差别采用单因素方差分析比较。结果:实验组的细胞培养24h后,在激光共聚焦显微镜下细胞质可见发光的荧光点,细胞核的光强度较弱。各组克隆形成率均P<0.05有显著差异。结论:石墨烯容易渗透到细胞质但没有进入细胞核,可以猜测石墨烯在进入活的滑膜间充质干细胞后没有发生遗传的中断。

Objective：To study the transfection efficiency of water soluble grapheme on synovuial stem cells.Methods：The third generation of synovial-derived mesenchymal stem cells（SMSCs）was divided into two groups.In experimental group,cells were cultured with the concentration of 25moL/mL water soluble grapheme for 24 hours at the temperature of 37℃.For the control group,cells were cultured with common medium for 24 hours at the temperature of 37℃.The laser scanning microscope with 405 nm excitation wavelength was employed to detect the cells marked by the water soluble grapheme.Results：Fluorescence spots could be observed in the cytoplasmic area of these stem cells in experimental group 24 hours later under the laser scanning microscope,and the light intensity of nucleus was relatively weak.Conclusion：Grapheme quantum dots（GQDs）are easily penetrated into the cytoplasm but not the nucleus.It is suggested that genetic disruptions might not happen after the GQDs entered the living stem cells.