1H NMR代谢组学方法对牙周炎患者唾液代谢轮廓的分析

Analysis of Saliva Metabolic Profile of Patients with Periodontitis using 1H NMR Metabolomics Method

目的:运用基于氢谱核磁共振(1H-NMR)的代谢组学方法分析牙周炎患者的唾液,寻找差异代谢物。方法:通过血常规检测选取符合条件的受试者作为实验组和对照组;收集唾液进行核磁共振检测,结合代谢图谱,利用模式识别分析得出区分健康个体和牙周炎患者贡献较大的差异性变量,将同时满足VIP>1.0和单维统计P<0.05的代谢物确定为最终的差异代谢物。结果:基于氢谱核磁共振的代谢组学方法可以区分牙周炎患者和健康个体。最终的差异代谢物有葡萄糖、丁酮酸和亮氨酸。结论:这些差异代谢物的变化可能会对牙周炎的临床研究提供实验依据。代谢组学方法有望在早期牙周炎的诊断中成为一种新的检测手段。

Objective：To discover the distinguishing metabolites in saliva of patients with periodontitis using 1H NMR-based metabolomics method.Methods：The subjects were selected as the experimental group and the control group by blood routine test.The saliva was collected and nuclear magnetic resonance was performed to identify the different metabolic profiles.The metabolites with VIP〉1 and univariate statistic P〈0.05 were identified as the ultimate distinguishing metabolites.Results：The metabolites glucose,threonine,and leucine were identified as potential indicators of periodontal inflammation.Conclusion：The metabolomics method has the potential to facilitate the screening and early diagnosis of periodontitis.