Er:YAG激光联合次氯酸钠对粪肠球菌杀菌效果的研究

Antimicrobial Effects of Enterococcus Faecalis in Root Canal using Er:YAG Laser Combined with Different Concentrations of Sodium Hypochlorite Irrigants

目的:比较掺铒钇铝-石榴石脉冲(Er:YAG)激光联合不同浓度次氯酸钠对根管内粪肠球菌杀菌效果的研究。方法:选择新鲜拔除的人单根管离体前磨牙建立粪肠球菌根管感染模型75个,随机分为5组,每组15个,分别进行如下处理:A组:5.25%次氯酸钠单独冲洗;B组:Er:YAG激光+5.25%次氯酸钠冲洗;C组:1%次氯酸钠单独冲洗;D组:Er:YAG激光+1%次氯酸钠冲洗。E组:空白对照。采集5组根管内的细菌进行培养计数并对结果进行卡方检验(Chi-square test)。结果:A、B、D三组均能完全杀灭根管内的粪肠球菌;C组未能完全杀灭根管内的粪肠球菌。15个培养皿中有11个可见粪肠球菌菌落。A、B、D三组间杀菌效果无差异且均显著优于C组。结论:高浓度次氯酸钠可以完全杀灭根管内的粪肠球菌,低浓度次氯酸钠不能完全杀灭根管内的粪肠球菌;Er:YAG激光可以显著增强低浓度次氯酸钠的杀菌作用。

Objective：To compare the antimicrobial effects of Er：YAG laser combined with different concentrations of sodium hypochlorite（NaClO）irrigants on enterococcus faecalis.Methods：Seventy-five single-root teeth were selected and contaminated with enterococcus faecalis for 4 weeks.The models were randomly divided into 5groups：5.25 % NaClO（group A）,Er：YAG laser＋ 5.25 % NaClO（group B）,1 % NaClO（group C）,Er：YAG laser＋ 1 % NaClO（group D）,and untreated（group E）.Group E was used to evaluate the success of models and the baseline number in the root canal.The amount of bacteria in the root canal of all models after treatments were measured by counting the colony-forming units（CFU）in the culture dishes and bacteria reduction was analyzed by means of Chi-square test.Results：Group A,B and D showed great antimicrobial effects and reduced the CFU to0.But group C could not kill all enterococcus faecalis.Four of 15 culture dishes of group C were empty and without any bacterial colony,but the rest were not.The antimicrobial effects between group A,B and D were the same and significantly better than group C.Conclusion：Low concentrations of NaClO do not have ability to kill all enterococcus faecalis but high concentrations of NaClO shows great antimicrobial effects.Er：YAG laser combined with low concentrations of NaClOirrigants has strong effects on disinfection of enterococcus faecalis.