食用菌提取物对胰岛素抵抗细胞体外糖代谢的影响

Effect of Different Edible Fungi on the Bioactivity of Anti-Insulin Resistance in Insulin Resistant Cells

以高胰岛素诱导的HepG2细胞和地塞米松诱导的3T3-L1脂肪细胞两种胰岛素抵抗细胞模型,分别对9种食用菌(桑黄、灵芝、香菇、杏鲍菇、鸡腿菇、灰树花、猴头、姬松茸和蛹虫草)醇提物、桑黄和灵芝的4种有机溶剂(正丁醇、乙酸乙酯、氯仿和石油醚)萃取物的体外降糖活性进行了研究。HepG2模型研究结果表明,9种食用菌醇提物中灵芝和桑黄醇提物促进细胞葡萄糖消耗效果最好,并且两者高浓度组的葡萄糖消耗量均好于阳性对照组;桑黄3种有机溶剂萃取相(正丁醇相、乙酸乙酯相和石油醚相)和灵芝4种有机溶剂萃取相促进细胞葡萄糖消耗效果较好,均好于阳性对照组。3T3-L1细胞模型结果表明,9种食用菌醇提物中灵芝和桑黄醇提物的促进葡萄糖消耗效果最好,均好于阳性对照组;灵芝及桑黄4种有机溶剂萃取相中,乙酸乙酯萃取相的葡萄糖消耗效果较好。

The effect of nine different edible fungi ethanolic extracts （Phellinu,s baumii, Gan.oderma lucidum, Cordyceps militaris ,Agaricus blazei, Coprinus comatus ,Lentinula edodes, Grifola frondosa, Hericium erinaceus and PleurotTts errngii） on glucose consumption was investigated in two models of insulin-resistant cells （high concentration insulin-induced HepG2 and dexamethasone-induced 3T3-LI ）. The glucose consumption of alcoholic extracts from the edible mushroom using four different organic solution （petroleum ether, chloroform ,ethyl acetate and n-butanol phase） from G. Lucidum or I： Baumii was detected in the two cell models. The cell glucose consumption was greatly enhanced by the ethanolic extracts from G. Lucidum or P. b（mrnii in HepG2 model. A better result of glucose consumption was achieved for the two groups with high-concentration compared with the positive control. The cell glucose consumption was also effectively promoted by P. ba^tmmi extracted by petroleum ether, ethyl acetate or n-butanol, and similar improvement was observed in G. Lucidum extracted with four types of organic solvents. The glucose consumption increased by ethanolic extracts from G. Lucidum and P. l^aumii in the study of 3T3-L1 adipocyte cell model. The extracts from P. bcutmrni and G. Lucidum by ethyl acetate facilitated the glucose consumption.