CD44s基因蛋白在非小细胞肺癌中的表达

Expression of CD44s protein in non-small cell lung cancer

目的 探讨标准型CD44(CD44s)基因蛋白在非小细胞肺癌(NSCLC)中各组织学类型的表达及与淋巴结转移的关系。方法 采用微波免疫组织化学染色方法(LSAB)检测CD44s在78例NSCLC中的阳性表达率。结果NSCLC总阳性表达率为74.4％(58/78),鳞癌、腺癌、大细胞癌和腺鳞癌的阳性率分别是63.2％(24/38)、88.9％(24/27)、83.3%(5/6)和71.4%(5/7),鳞癌与腺癌、大细胞癌之间有非常显著差异(P<0.01)。高、中、低和未分化组的阳性率分别是68.4％(13/19)、75.8％(25/33)、76.2％(16/21)和80.0％(4/5),高分化组和未分化组之间有显著性差异(P<0.05),其他各组之间无显著性差异(P>0.05)。转移和无转移的阳性率分别为48.1％(17/27)和88.2%(45/51),其中强阳性率分别是25.9％(7/27)和74.5%(38/51),不论是阳性率还是强阳性率,二组差异均非常显著(P<0.01)。结论 CD44s阳性表达率高、阳性强度强的病人转移率低,CD44s可作为区分NSCLC组织学类型及淋巴结转移潜能的辅助指标。

Objective To investigate the expression of standard CD44 ( CD44s) genes protein in non-small cell lung cancer (NSCLC) and its relation to the tumor's histology, differentiation and lymphatic metastasis. Methods Expression of CD44s was detected with microwave and LSAB immunohistochemistry in 78 cases of NCLCS. Positive rate of CD44s was correlated with their his-topathology, differentiation and lymphatic metastasis. Results Total positive rate was shown to be 74.4% (58/78) in NSCLC. Squamous cell carcinomas had a positive rate of 63. 2% (24/38) ;adenocarcinomas, 88. 9% (24/27) ;large cell carcinomas,83. 3% (5/6) and aclenosquamous carcinoma,? 1. 4% (5/7)respectively. There was a significant difference( P <0. 01) among the squamous cell and adenosquamous, and large cell carcinoma. The positive rates in highly. moderately, poorly differentiated and undifferentialed carcinomas were 68. 4% ( 13/19) ,75. 8% (25/33) ,76.2% ( 16/21 ) and 80.0% (4/5) respectively. Significant difference ( P < 0. 01) existed between highly differentiated and undifferenlialed carcinomas. Total and strong positive rates of NSCLC were 48. 1% (17/27)and 25. 9% (7/27)in the cases with lymphatic metastasis, while 88. 2% (45/51 ) and 74. 5% (38/51 ) in those without metastasis (P < 0. 01). Conclusions NSCLC patients with higher CD44s positive expression had lower metastasis. The expression of CD44s in cancer cell could be used as a valuable marker in verifying the histological classification and the potentials of lymphatic metastasis.