益心脑滴丸对肾性高血压大鼠的降压作用及其机制研究

Antihypertensive effect of Yixinnao dripping pills on renal hypertensive rats and its mechanism

目的探讨益心脑滴丸对肾性高血压大鼠(RHR)的降压作用及机制。方法构建两肾一夹(2K1C)经典肾性高血压大鼠模型,选取造模成功的大鼠并按收缩压水平分为模型组,益心脑高、中、低剂量组(2,1,0.5 g·kg^(-1)·d^(-1))及卡托普利组(25 mg·kg^(-1)·d^(-1)),同时设立假手术组,每组8只。灌胃给药每日1次,每周末测量血压,6周后处死并检测血浆内皮素(ET)和一氧化氮(NO),总抗氧化能力(T-AOC)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和肾组织活性氧(ROS)、谷胱甘肽过氧化物酶(GSH-Px)的变化;用酶联免疫法(ELISA)检测血浆肾素活性(RA)及血管紧张素Ⅰ(AngⅠ),AngⅡ的含量。结果益心脑滴丸能明显降低高血压大鼠收缩压(SBP)(P<0.05);增加血浆NO,降低ET含量(P<0.05);降低ROS和MDA的含量,提高抗氧化酶SOD,CAT和GSH-Px活性(P<0.05),使T-AOC增强;升高血浆AngⅠ含量,降低血浆AngⅡ含量(P<0.05)。结论益心脑滴丸有一定的降压作用,其机制可能与抗氧化,影响肾素-血管紧张素系统有关。

Objective To investigate the antihypertensive effect of Yixinnao dripping pills on renal hyperten-sive rats （RHR） and its mechanism. Methods The two-kidney one-clip （2K1C） goldblatt RHR models were estab-lished. The successfully established rat models were randomly divided into the model group, high, medium and low dose Yinxinnao groups （2, 1, 0.5 g·kg^-1·d^-1） and captopril group （25 mg·kg^-1·d^-1）, and the sham operation group was established （n=8 each）. All the rats received intragastric administration once daily and the blood pressure determina-tion every weekend, and were sacrificed at 6 weeks. The changes of plasma endothelin （ET）, nitric oxide （NO）, total anti-oxidation capability （T-AOC）, malondialdehyde （MDA）, superoxide dismutase （SOD）, catalase （CAT）, reactive oxy-gen species （ROS） of renal tissues, and glutathione peroxidase （GSH-Px） were determined. The levels of plasma renin activity （RA）, angiotensin Ⅰ, Ⅱ （Ang Ⅰ, Ang Ⅱ） were measured by enzyme-linked immunosorbent assay （ELISA）. Results Yixinnao dripping pills could significantly decrease the systolic blood pressure （SBP） of hypertensive rats （P〈0.05）, increase the plasma NO and decrease the ET level （P〈0.05）. T-AOC could be enhanced by decreasing the levels of ROS and MDA, and increasing the activities of antioxidant enzymes SOD, CAT and GSH-Px （P〈0.05）. The level of plasma Ang I was increased, and the plasma Ang Ⅱ was decreased （P〈0.05）. Conclusion Yinxinnao drip-ping pills may have a certain antihypertensive effect, and its mechanism may be related to antioxidant, affecting the renin-angiotensin system.