CuFe_2O_4纳米粒子增敏Luminol-EDTA化学发光体系研究及应用

Enhancement of chemiluminescence of Luminol-EDTA system by CuFe_2O_4 nanoparticals and its application

基于CuFe_2O_4纳米粒子能显著增强Luminol-EDTA体系的发光,首次建立了Luminol-EDTA-CuFe_2O_4 NPs化学发光新体系。紫外吸收光谱和化学发光光谱表明纳米CuFe_2O_4注入Luminol-EDTA体系后,未生成新发光物质,结合纳米CuFe_2O_4的特性,提出了CuFe_2O_4 NPs参与Luminol-EDTA体系可能的发光机理。研究发现芦丁能抑制Luminol-EDTACuFe_2O_4 NPs体系的化学发光,结合流动注射技术,将此化学发光体系应用于芦丁片中芦丁含量的测定。在优化实验条件下,芦丁浓度在2×10^(-8)~2×10^(-5) mol/L范围内芦丁浓度的对数和相对化学发光值呈线性,芦丁浓度检出限(LOD)为1.21×10^(-9) mol/L。将本方法应用于芦丁片中的芦丁含量测定,回收率为97%~102%,RSD为2.54%(c=1×10^(-7) mol/L,n=11)。

A new chemiluminescence system of Luminol-EDTA-CuFe204 NPs was firstly developed based on the fact that CuFe204 nanopartieals （NPs） could significantly enhance the chemiluminescence （CL） intensity of the Luminol-EDTA system. The UV-Vis spectra and the chemiluminescence spectra suggested that no new luminophor formed after the introducing of CuFe2 04 NPs in the Luminol-EDTA system. The possible mechanism of CuFe2O4 NPs participated in Luminol-EDTA system was proposed by combining with the property of CuFe2O4 NPs. Additionally, it was found that rutin could inhibit the chemiluminescence of the proposed Luminol-EDTA-CuFe2O4 NPs system. Combined with flow injection, the system was applied to the determination of turin in rutin tablets. Under the optimal conditions, the relationship between the relative CL intensity and the logarithm of concentration of rutin was linear when the concentration of ratin ranged from 2× 10^-8 mol/L to 2 × 10^-5 mol/L with the detection limit of 1.21× 10^-9 mol/L. The recommended method has been successfully applied to the determination of rutin tablets and the recovery was between 97%-102% and RSD （c=1×10^-7mol/L , n=ll） was 2.54%.