HPLC-MS-MS法测定养殖水体中18种磺胺类药物的残留量

Determination of 18 Kinds of SAs in Aquaculture Water by HPLC-MS-MS Method

[目的]应用固相萃取(SPE)及高效液相色谱-串联质谱(HPLC-MS-MS)分析技术,建立养殖水体中的18种磺胺类药物残留量测定方法。[方法]水样中的磺胺类药物通过HLB萃取小柱富集后,以C18反相柱为分析柱,0.1%甲酸水溶液和乙腈为流动相,采用HPLC-MS-MS进行定量分析。[结果]HPLC-MS-MS选择多反应监测(MRM)正离子模式测定,可同时对养殖水体中的18种磺胺类药物进行定性和定量。磺胺类药物的检出限均低于1.0 ng/m L,方法的平均回收率为70%~120%。[结论]该方法能够简便、快捷、有效地实现养殖水体中18种磺胺类药物残留的快速检测。

Using solid phase extraction （SPE） and HPLC-MS-MS analysis technology,determination method for 18 kinds of sulfa drugs residues in aquaculture water was established. [ Method] Sulfa drugs in water sample were enriched through HLB extraction column,with C18 reversed-phase column as analytical column,0. 1% formic acid aqueous solntion and acetonitrile as mobile phase,using HPLC-MS-MS to con- duct quantitative analysis. [ Result ] Applying HPLC-MS-MS selective reaction monitoring （SRM） positive ion mode to determine, at the same time qualitative and quantitative analysis of 18 kinds of SAs in aquaculture water was conducted. The detection limits of SAs were less than 1.0 ng/mL, the range of the average recovery was 70% -120%. [ Conclusion] The method is simple ,quick and effective to realize rapid detection of 18 kinds of sulfa drugs residues in aquaculture water.