摘要
目的:比较mini-VIDAS法、膜芯片2种快速筛检方法和国标培养法,评估2种快速筛检方法的效果和可操作性。方法:以鼠伤寒沙门氏菌为目标菌,针对3种筛检方法设计灵敏性、特异性、抗干扰性试验和人工污染试验。结果:国标培养法和mini-VIDAS法的灵敏性较好,miniVIDAS法和膜芯片法的特异性和抗干扰性较好,人工污染试验三者表现符合设计预期。结论:日常检验中可采用国标培养法和mini-VIDAS法进行筛检工作,应急任务可使用膜芯片法缩短筛检时间,mini-VIDAS法和膜芯片法是沙门氏菌快速筛检的可靠工具。
Objective： This study aimed to compare of three pre-detection methods for detection of Salmonella. Methods： Experiments were designed to test the sensitivity, specificity and anti interference test of these methods, artificially contaminated samples were tested and verified. Results： National standard and mini-VIDAS method with high sensitivity, mini-VIDAS and membrane-based array method with specificity and high anti-interference, artificially contaminate test was as expected. Conclusion： National standard and mini-VIDAS methods can be used for pre-detection in daily inspections, membrane-based array method could shorten the pre-detection time in emergency testing. Additionally, mini-VIDAS and membrane-based array are good tools for pre-detection of the Salmonella.
出处
《食品科技》
CAS
北大核心
2017年第4期306-309,共4页
Food Science and Technology
基金
重庆市社会民生科技创新专项(cstc2015shmszx00002)
