解整合素-金属蛋白酶33在不同严重程度哮喘患者血清中的表达水平及其与气道慢性炎症的关系

Expression of serum ADAM33 in patients with different severity of asthma and the relation to airway chronic inflammation

目的解整合素-金属蛋白酶33(ADAM33)是哮喘重要的易感基因之一,与哮喘发病和气道高反应性密切相关。文中评估ADAM33在不同严重程度哮喘患者血清中表达水平及其与气道慢性炎症的关系。方法回顾性分析2015年5月1日至2016年5月1日期间于南京总医院就诊的轻-中度哮喘(n=54)、重度哮喘(n=35)和对照组(n=30)患者,分别检测血清总Ig E水平、外周血嗜酸性粒细胞(EOS)计数和血清中ADAM33、IL-4和IL-13表达水平,并进行相关性分析。结果轻-中度哮喘组、重度哮喘组和对照组患者血清中ADAM33表达分别为(23.8±6.21)、(64.8±12.8)和(18.3±4.49)pg/m L,重度哮喘组和轻-中度哮喘组血清ADAM33表达均显著高于健康对照组,且重度哮喘组血清ADAM33表达也同样高于轻-中度哮喘组(P<0.05)。相关分析结果显示,ADAM33表达与IL-4和IL-13呈正相关性(r=0.79、r=0.81,P<0.05),而与EOS计数和血清总Ig E无相关性(r=0.54、r=0.46,P>0.05)。结论 ADAM33在不同严重程度哮喘患者血清中的升高可反映哮喘的严重程度。血清IL-4和IL-13表达水平与ADAM33的正相关提示ADAM33的表达水平可能受到Th2类细胞因子的调控。

Objective Adistintegrin and metalloproteinase 33（ ADAM33） is one of the asthma susceptibility gene,which is closely related to the pathogenesis of asthma and airway hyperreactivity. The aim of this study was to evaluate the expression of serum ADAM33 in patients with different severity of asthma and the relation to airway chronic inflammation through clinical trials. Methods Patients diagnosed as mild-to-moderate asthma（ n = 54）,severe asthma（ n = 35） and healthy controls（ n = 30） were recruited from May 2015 to May 2016. The serum Ig E,ADAM33,IL-4 and IL-13 1evels and Eosinophil（ EOS） in peripheral blood were detected,and the correlation analysis was performed Results The serum levels of ADAM33 in mild-to-moderate asthma group,severe asthma group and healthy control group were 23. 8 ± 6. 21pg/m L,64. 8 ± 12. 8pg/m L and 18. 3 ± 4. 49pg/m L,respectively. The ADAM33 levels in mild-to-moderate asthma group and severe asthma group were higher than those in control group（ P〈0.05）. The ADAM33 levels in severe asthma group were higher than those in mild to moderate asthma group.（ P〈0.05）. The correlation analysis showed that ADAM33 had positive correlation with IL-4 and IL-13（ r = 0. 79 and r = 0. 81）,but no correlation with EOS and Ig E（ r = 0. 54 and r = 0. 46）.Conclusion The expression of serum ADAM33 was up-regulated in asthmatic patients along with the severity of asthma. ADAM33 was positively correlated with serum IL-4 and IL-13,implying that the expression of ADAM33 may be regulated by Th2 type cytokines.