麝香对颅骨骨缺损模型大鼠FGF-2和EGF mRNA表达的影响

Effects of musk on mRNA expressions of FGF-2 and EGF in the rat model of skull bone defect

目的探讨麝香对颅骨骨缺损模型大鼠成纤维细胞生长因子2(fibroblast growth factor,FGF-2)和表皮生长因子(epidermal growth factor,EGF)表达的影响。方法 300只SD大鼠(雌、雄各150只)通过牙科钻建立颅骨骨缺损模型,模型动物采用完全随机方法分为模型组和给药组(每组150只),两组再按照给药时间各分为7、14、28d3个小组,每组50只。给药组每天灌服天然麝香(4.2mg/100g),模型组每天灌服等量的生理盐水,采用实时荧光定量PCR法测定颅骨骨缺损处FGF-2mRNA和EGF mRNA的表达。结果给药组在第7天和第14天EGF mRNA表达水平均高于模型组,但均无统计学差异,第28天EGF mRNA表达水平降到最低,与模型组比较差异有统计学意义(P<0.05);给药组在第7天FGF-2mRNA表达最高,与模型组比较差异有统计学意义(P<0.05),第14天和第28天降低,与模型组比较无显著差异。结论麝香在不同时间段对大鼠颅骨骨缺损区的愈合有不同的影响,其机制可能与上调FGF-2mRNA表达和下调EGF mRNA表达水平有关。

Objective To study the effects of musk on the expressions of fibroblast growth factor 2(FGF-2)and epidermal growth factor(EGF)in the rat model of skull bone defect.Methods We constructed the bone defect model by dental drilling into the full skull of 300 SD rats(150 males and 150 females).The model animals were divided with completely random method into model group and drug group,with 150 in each.The two groups were further divided according to drug administration time into7,14 and28 d groups,respectively,with50 in each.The drug group received perfusion of natural musk every day(4.2 mg/100 g)while the model group received perfusion of normal saline of the same volume every day.FGF-2 mRNA and EGF mRNA expressions in skull bone defect were determined using Real-time fluorescent quantitative PCR method.Results EGF mRNA expression at7 d and 14 d was higher in the drug group than in the model group,but with no significant difference.EGF mRNA expression at 28 d decreased to the lowest level,with a significant difference(P<0.05).FGF-2 mRNA expression in the drug group reached the highest at 7 d,with a significant difference(P<0.05),and decreased at 14 and 28 d without significant difference.Conclusion Musk administered at different time points can effectively promote the healing rate of the bone defect area of the rat skull,and the mechanism of this repair is mainly related to the increased FGF-2 mRNA expression and the decreased EGF mRNA expression.