红霉素分子印迹二维光子晶体水凝胶传感器的研究

Erythromycin Molecularly Imprinted Two-dimensional Photonic Crystal Hydrogel Sensor

以红霉素为印迹分子,聚苯乙烯二维光子晶体为模板,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸甲酯为交联剂,2,2-二乙氧基苯乙酮为引发剂,紫外光引发聚合,在甲醇-乙酸(9:1,V/V)中洗脱印迹分子,得到能够特异性识别红霉素的分子印迹二维光子晶体水凝胶。通过测试德拜环直径变化,研究了此水凝胶在红霉素溶液中的响应性能。实验结果表明,当红霉素的浓度从0增加到1×10^(-6)mol/L时,德拜环直径增加6 mm,相应的晶格间距减小30 nm。此外,水凝胶在1×10^(-6)mol/L红霉素的类似物罗红霉素、琥乙红霉素溶液中,德拜环直径仅分别增加1.5和2.0 mm,表明此光子晶体水凝胶具有良好的选择性,有望用于红霉素低成本的简易检测。

A molecularly imprinted two -dimensional photonic crystal hydrogel sensor was developed with erythromycin as imprinted molecule, polystyrene two-dimensional photonic crystal as templates, methanol as solvent, methacrylic acid as monomers and ethylene glycol dimethylacrylate as cross- linkers. The imprinted molecule was removed by methanol/acetic acid （9：1,V / V） . The results showed that the diameter of Debye ring increased 6 mm when the concentration of EM changed from 0 to 1 X10＂6 mol/L. Namely the lattice spacing decreased 30 nm. In addition, the diameter of Debye ring only increased 1.5 and 2. 0 mm when the hydrogel immersed in 1X10-6 mol/L roxithromycin （RM） or erythromycin ethylsuccinate （EEs） solution. The result indicated that the sensor had high selectivity and could be used in determination of erythromycin with low cost and easy operation.