2013-2015年中国冬油菜参试品种(系)DNA指纹数据库的初步构建及分析

Construction and analysis of DNA fingerprint database of winter rapeseed in China from 2013 to 2015

为了满足日益增长的冬油菜新品种鉴定需要,采用多色SSR荧光标记组合毛细管电泳检测法,构建了419份2013-2015年度国家冬油菜区域试验中的冬油菜新品种(系)×38对SSR荧光标记的DNA指纹数据库。所有标记共检测到41个位点,其中双位点标记3个,单位点标记35个。38个多态性SSR标记于419份冬油菜新品种中共检测到129个等位变异;每个位点的变异范围为2~6,平均每个位点3.15个。等位基因型数共250个,每个位点的基因型数为3~15个,平均每个位点6.09个。41个位点的多态性信息指数PIC值变幅为0.132 2~0.690 7,平均为0.390 2。统计指纹数据库缺失率为1.1%,具有较好的完整性。DICE相似系数矩阵分析得出,419份中国冬油菜新品种(系)间成对遗传相似系数共获取87 571个数据项,品种间遗传相似系数变幅为0.366~0.983,平均遗传相似系数为0.692。相似系数在区间0.601~0.800内共有78 571个数据项,占总数的89.72%。此结果一定程度表明冬油菜新品种(系)的遗传多样性不够丰富。

To meet the future demand of winter rapeseed identification, a DNA fingerprint database containing genotypic data of 419 new winter rapeseed varieties （lines） ×38 markers were constructed by capillary electropho-resis detection method with fluorescent SSR markers. 41 locus and 129 alleles were detected by 38 fluorescent SSR primers on 419 newly bred winter rapeseed varieties （lines） . Among the 38 markers, 35 were for 1 locus and 3 for 2 loci. The number of alleles per loci ranged from 2 to 6 with an average of 3. 15 per locus. The number of geno-type were 250 and varied from 3 to 15, with an average of 6. 09 per locus. The polymorphic information content values ranged from 0. 132 2 to 0. 690 7 with an average of 0. 39. Missing statistics calculated fingerprint database at the rate of 1. 1 %. 87 571 data items were obtained by comparing DICE similarity coefficient among varieties （lines）. DICE similarity coefficient among varieties （lines） ranged from 0. 366 to 0. 983 with an average of 0. 692. Within the DICE similarity coefficient range from 0. 601 to 0. 800, there were 78 571 data items, which ac-counted for 89. 72% of the total. The results showed that these winter rapeseed varieties （lines） had limited genet-ic diversity.