不同品种亚麻籽木酚素多聚体水解物的组成及含量

Lignan content and its hydrolysates of flaxseeds in different cultivars

为考察国内主栽品种亚麻籽的木酚素含量,采用反相高效液相色谱技术,测定分析了24个品种的亚麻籽木酚素多聚体的水解产物。结果表明:亚麻籽木酚素碱水解产物主要为对香豆酸糖苷(CouAG)和开环异落叶松酚葡萄糖苷(SDG),酸水解产物主要为开环异落叶松脂素(SECO)、对香豆酸(CouA)和5-羟甲基-2-糠醛(HMF)单体,其中含量最高的组分为SDG。不同品种和产区的亚麻籽木酚素水解产物的含量具有显著差异(P<0.05)。24个品种亚麻籽中,坝亚9号的对香豆酸糖苷(6.07±1.97mg CouA/g)、SDG(33.31±0.50 mg/g)、SECO(5.03±0.16mg/g)和对香豆酸(0.87±0.01mg/g)含量最高,内亚6号的HMF含量最高达1.63±0.03mg/g,而定亚23号的对香豆酸糖苷(1.16±0.15mg CouA/g)、SDG(11.37±0.77mg/g)和对香豆酸(0.18±0.01mg/g)含量最低,内亚9号的SECO含量低于1mg/g(0.76±0.01mg/g),轮选2号的HMF含量最低为0.13mg/g。6个亚麻产区中内蒙古、山西和河北地区的亚麻籽木酚素水解产物的平均含量相对较高。

To determine the contents of lignans and its components in flaxseed with different cultivars in China, 24 cultivars of flaxseed samples were collected from the main producing areas （including Inner Mongolia, Gansu, Shanxi, Hebei, Ningxia, Xinjiang）, and the content of lignan and its components were determined by HPLC. The results showed that the alkaline hydrolysates of flaxseed lignans were p-coumaric acid glycoside and SDG; and the acid hydrolysates were SECO, p-coumaric acid and HMF. SDG was the highest in flaxseed lignans. The content of lignans hydrolyzate of flaxseed in different cultivars and areas was significantly different （P 〈0.05）. Among 24 cultivars of flaxseed samples, the contents of CouAG （6.07 ± 1.97mg CouA/g）, HMF （33.31 ± 0.50 mg/g）, SECO （5.03 ± 0.16 mg/g ） and CouA （0.87 ± 0.01 mg/g） in Baya 9 were the highest, the contents of CouAG （1.16 ± 0.15 mg CouA/g ）, SDG （11.37 ± 0.77 mg / g ） and CouA （0.18 ± 0.01mg/g） in Dingya 23 were the lowest. The content of SECO in Neiya 9 was lower than 1 mg/g seed （0.76 ± 0.01 mg/g ）, and the content of HMF in Lunxuan 2 was the lowest at 0.13 mg / g . The content of SDG and its hydrolysates of flaxseed in Inner Mongolia, Shanxi and Hebei was relatively higher among the 6 flaxseed producing areas.