尖锐湿疣皮损中Caspase8与P-Akt的表达

Expression of Caspase 8 and phospho-Akt in condyloma acuminatum lesions

目的 探讨Caspase 8与p？Akt在尖锐湿疣皮损中的表达及意义。方法 收集尖锐湿疣30例、宫颈癌20例、正常皮肤（包皮）20例，采用免疫组化检测以上组织中Caspase 8及p？Akt的表达及分布。结果 Caspase 8在尖锐湿疣皮损、宫颈癌、正常皮肤中阳性率分别为23.33%、80%、90%。Caspase 8在尖锐湿疣中的表达强度显著低于正常皮肤（P＜0.01）和宫颈癌组织（P＜0.001）。p？Akt在尖锐湿疣皮损、宫颈癌组织、正常皮肤组织中的阳性率分别为93.33%、95%、90%。p？Akt在尖锐湿疣皮损的表达强度显著高于正常皮肤组织（P＜0.001），低于宫颈癌组织（P＜0.001）。在尖锐湿疣皮损和正常皮肤组织中，Caspase 8与p？Akt的表达无相关性。在宫颈癌中，Caspase 8与p？Akt的表达存在显著的正相关（r = 0.369，P＜0.05）。结论 Caspase 8的凋亡启动受抑制及p？Akt的抗凋亡作用可能参与了尖锐湿疣的发生发展。

Objective To determine the of Caspase 8 and phospho-Akt （p-Akt） in condyloma acuminatum （CA） lesions, and to evaluate their significance. Methods Skin lesion samples were collected from 30 patients with CA, cancer tissue samples from 20 with cervical cancer, and normal skin samples from 20 healthy controls. All the samples were subjected to paraffin embedding. An immunohistochemical study was conducted to determine the and distribution of Caspase 8 and p-Akt in the above samples. Results The rate of Caspase 8 was significantly lower in CA lesions （23.33%） than in normal skin samples （90%, P 〈 0.01） and cervical cancer lesions （80%, P 〈 0.001）. Moreover, the rate of p-Akt in CA lesions （93.33%） was significantly higher than that in the normal skin samples （90%, P 〈 0.001）, but lower than that in the cervical cancer lesions （95%, P 〈 0.001）. No significant correlations were observed between the of Caspase 8 and p-Akt in either CA lesions or normal skin samples. However, the of Caspase 8 was positively correlated with the of p-Akt in cervical cancer lesions （r = 0.369, P 〈 0.05）. Conclusion Both suppressed apoptosis initiation of Caspase 8 and anti-apoptotic effect of p-Akt may be involved in the occurrence and development of CA.