水稻细菌性条斑病的广谱抗性资源筛选

Screening of broad-spectrum resistant resources against rice bacterial leaf streak

【目的】筛选在多个生育期对多个强致病力细菌性条斑病病菌具有广谱抗性的水稻材料,为水稻细菌性条斑病抗性品种的培育提供可靠抗源材料。【方法】以1100份具有丰富遗传背景的水稻品系为试验材料,以高感病品种金刚30为感病对照,以5株强致病力水稻细菌性条斑病病菌为接种对象,采用针刺法进行多生育期、多个强致病力细菌性条斑病菌菌株重复接种抗性筛选鉴定。【结果】初筛获得14份抗病材料,占全部材料的1.27%。复筛获得9份具有中抗级别以上的材料,占全部材料的0.82%,其中有3份材料(RL6、RL9和RL14)在水稻的3个生育期对多株水稻细条病菌菌株具有抗性,尤其是RL6表现出对多菌株的广谱抗性,且抗性水平高;6份材料(RL2、RL4、RL5、RL8、RL11和RL12)在单个生育期对单株水稻细条病菌菌株具有抗性。【结论】获得3份对水稻细菌性条斑病具有多生育期广谱抗性的材料,可作为重要的抗源应用于水稻抗性品种培育,其中RL6可作为优质抗源优先应用于水稻抗性品种培育;获得6份在特定生育期对水稻细菌性条斑病具有抗性的材料,可作为候选抗源应用于水稻抗性品种培育。

[Objective]Rice lines with broad-spectrum resistance toward several kinds of pathogenic bacteria of Xan-thomans oryzae pv. Oryzicola at multiple growth stages were selected in order to provide source for rice bacterial leaf streak resistance breeding. [Method]A total of 1100 rice lines with rich genetic background were offered as selective objects. Highly susceptible cultivar Jingang 30 was used as control. Inoculation was carried out on five strong pathogenic bacteria. Acupuncture method was used in resistance identification using multiple pathogenic strains at various growth stages. [Re-sult]Fourteen disease-resistant materials were obtained through preliminary screening , accounting for 1.27% of the total materials. Nine materials over mediately resistant were obtained via secondary screening , accounting for 0.82% of the total materials. Besides, three of them（RL6,RL9 and RL14） were resistant to a number of bacterial leaf streaks at three growth stages. In particular, RL6 presented broad-spectrum resistance to multi-strains with high resistance levels. Six of them （RL2、RL4、RL5、RL8、RL11 and RL12） were resistant to single strain at single growth period. [Conclusion]The obtained three materials are broad-spectrum resistant to rice bacterial leaf streak at multiple growth periods , which can be served as an important source for cultivating disease-resistant rice varieties. RL6 maintains high broad-spectrum resistance at all growth stages, which can be used as a prior resistance source for breeding rice varieties. While the six materials obtained with resistance at particular growth stages can be used as alternative resistance sources.