摘要
利用静电纺丝和模拟体液仿生矿化技术制备了聚乳酸-羟基乙酸共聚物/柞蚕丝素/羟基磷灰石((PLGA/TSF/HA)骨组织工程复合支架。通过扫描电子显微镜(SEM)、透射电子显微镜(TEM)、X射线衍射测试(XRD)和热重分析(TG)对复合纳米纤维的形貌结构进行了表征。此外,在复合纳米纤维支架材料上接种人骨髓间充质干细胞(hMSCs),通过四甲基偶氮噻唑蓝比色(Four methyl azo thiazole blue colorimetric,MTT)法,观察细胞在材料表面的生长情况评价纳米纤维的生物相容性。结果显示,PLGA/TSF纳米纤维毡具有精细的三维结构,纤维直径分布均匀,表面光滑。矿化后HA颗粒均匀地分布在PLGA/TSF纳米纤维表面,矿物含量约占63%。与PLGA/TSF纳米纤维支架相比,PLGA/TSF/HA纳米纤维支架的亲水性、生物相容性都得到显著提高。
PLGA/TSF/HA electrospun nanofibers for bone tissue engineer composite scaffolds were prepared by technologies of electrospinning and simulated body fluid biomimetic mineralization. The morphology and structure of composite nanofibers were characterized by SEM,TEM,XRD and TG.Besides,hMSCs were cultured in vitro to study the biocompatible properties of composite nanofibers,which were characterized by Four methyl azo thiazole blue colorimetric（MTT）and SEM.The results indicated that PLGA/TSF nanofibers have the refined three-dimensional structure and the uniform diameter distribution with the smooth surface. HA particles uniformly distributed on the surface of PLGA/TSF nanofibers after mineralization,and the content of mineral was about 63%.The hydrophily and biocompatibility of PLGA/TSF/HA nanofiber scaffolds were significantly enhanced compared with PLGA/TSF.
作者
王倩
邵伟力
高艳菲
何建新
陈利
崔世忠
WANG Qian SHAO Weili GAO Yanfei HE Jianxin CHEN Li CUI Shizhong(School of Textiles, Zhongyuan University of Technology, Zhengzhou 450007, China Key Laboratory of Advanced Textile Composites （Ministry of Education）, Institute of Textile Composites, Tianjin Polytechnic University, Tianjin 300387, China Collaborative Innovation Center of Textile and Garment Industry, Henan Province, Zhengzhou 450007, China)
出处
《材料科学与工程学报》
CAS
CSCD
北大核心
2017年第2期253-256,295,共5页
Journal of Materials Science and Engineering
基金
国家自然科学基金资助项目(51203196
U1204510)
NSFC-河南人才培养联合基金资助项目(U1204510)
河南省教育厅科学技术研究重点资助项目(14A540003
14A540006)
郑州市普通科技攻关资助项目(141PPTGG400)
关键词
聚乳酸-羟基乙酸共聚物
柞蚕丝素
羟基磷灰石
生物相容性
poly（lactic-co-glycolic acid）（PLGA）
tussah silk fibroin（TSF）
hydroxyapatite（HA）
Biocompatible properties
