ATRA对小鼠的急性毒性与抗感染作用研究

Acute Toxicity and Anti-infection Effect of ATRA in Mice

为研究全反式维甲酸(ATRA)对小鼠的急性毒性与抗感染作用,在ATRA急性毒性试验中采用最大给药量,记录小鼠给药7 d内的临床症状,然后通过剖检及病理组织学观察研究ATRA的急性毒性。将大肠杆菌经口感染小鼠建立感染模型,记录小鼠感染7 d内的临床症状,通过剖检和器官指数的测定来研究ATRA对小鼠的抗感染作用。结果表明,ATRA以最大给药量(人用剂量500倍)灌胃没有对小鼠造成明显的异常反应,体重与对照组相比差异不显著,剖检和病理组织学观察无可见病变。同时,口服高剂量ATRA的小鼠在感染后体重恢复效果好于低剂量组,口服ATRA的小鼠肝脏、肾脏、脾脏器官指数与对照组相比差异显著。说明ATRA对小鼠无急性毒性,同时具有良好的促进肝脏、肾脏、脾脏生长发育,提高机体免疫力和抗感染效果,可应用于兽医临床治疗以及疾病预防和控制。

In order to study the acute toxicity of all-trans retinoic acid （ATRA） and its anti-infection effect in mice, the maximum dosage was used in ATRA acute toxicity experiment, while the clinical symptoms of mice were recorded for 7 days. Then acute toxicity of ATRA was studied by autopsy and histopathology observation. The mice infection model was established by oral infection with Escherichia coli to study the anti-infection effect of ATRA by recording the clinical symptoms, autopsy lesions and organ index of infected mice for 7 days. The results showed that the mice had not abnormal response and pathological changes in autopsy and histopathological observation compared to the control group in the maximum dosage of ATRA （500 times of the clinical dosage in human） . The weight was also of insignificant difference compared to the control group. At the same time, the high dose of ATRA in mice to regain the weight was better than the low dose group before infection with Escherichia coll. Liver index, kidney index and spleen index by using ATRA were significantly different compared with control groups. ATRA had no acute toxicity in mice and was good for the growth of liver, kidney and spleen to improve the immunity and anti-infection effect, which can be used in clinical veterinary treatment and disease prevention and control.