血浆内脂素水平和组织表达状况与结直肠癌相关性研究

Study on the relationship between plasma lipid level and tissue expression and colorectal cancer

目的检测结直肠癌患者血浆内脂素水平和组织表达状况,并观察不同严重程度患者内脂素在血浆和组织中的表达,分析内脂素与结直肠癌的相关性。方法选择2014年1月至2016年9月结直肠癌患者约50例(研究组),同时选取50例健康成年人(正常组)及50例结直肠腺瘤患者(对照组),采用酶联免疫吸附试验(ELISA)方法测定血浆内脂素水平,应用免疫组化法检测组织中内脂素的表达,检测光密度OD值。结果研究组患者血浆内脂素为(16.38±1.06)μg/L,高于对照组(11.56±0.90)μg/L和正常组(5.93±0.84)μg/L,且对照组高于正常组,差异具有统计学意义(P<0.05)。研究组患者组织内脂素OD值为(44.59±7.19),高于对照组(31.24±6.30),差异具有统计学意义(P<0.05)。Dukes分期C期和D期、低分化血浆组织内脂素及OD值分别为(19.24±1.17)μg/L、(20.19±0.98)μg/L和(48.22±7.06)、(47.23±6.34),均高于A期和B期及中、高分化组(13.15±1.05)μg/L、(12.46±1.12)μg/L和(35.07±6.92)、(34.81±6.50),差异具有统计学意义(P<0.05)。结论结直肠癌、结直肠腺瘤患者血浆和组织内脂素水平均显著增高,且随着肿瘤分期增加、分化程度的降低而出现增高趋势,机体内脂素水平与结直肠癌呈现正相关。

Objective To detectthe plasma visfatin level and tissue expression in the patients with colorectal cancer, and to observe the expression of visfatin in plasma and tissues in patients with different severity.To analyze the he relationship betweenthe visfatin andcolorectal cancer. Methods 50 patients with colorectal cancer （studygroup）, 50 healthy aduhs （normal group） and 50 patients with colorectal adenoma （control group） were selected from January 2014 to September 2016.The content of visfatin in plasmawas detected by enzyme linked immunosorbent assay （ELISA） and the tissue was detected by immunohistochemical method andthe optical density （OD）. Results The level of plasma visfatin in the study group was （16.38±1.06）μg/L, which was significantly higher than that inthe control group （11.56±0.90） μg/L and normal group（5.93±0.84）μg/L, there was statistical significance （P〈0.05）. The OD value of visfatin in the study group was （44.59±7.19）, which was significantly higher than that inthe control group （31.24±6.30） , there was statistical significance （P〈0.05）.The visfatin levels plasma and tissue OD value of C and D stage in Dukes stage and poorly differentiated respectively （19.24 ± 1.17） μg / L, （20.19± 0.98）μg / L and （48.22 ± 7.06）, （47.23 ± 6.34） ,respectively higher than those of stage A and B,moderate differentiation and high differentiation were （13.15±1.05）μg/L, （12.46±1.12）μg/L and （35.07±6.92）, （34.81±6.50） respectively, there was statistical significance（P〈0.05）. Conclusion The visfatin levels of plasma and tissuein patients with colorectal cancer and colorectal adenoma were significantly increased, and and increased with the tumor stage increased and the degree of differentiation decreased.The levels of visfatin in the body hadthe positive correlation with colorectal cancer.