苦菜地上部分总黄酮富集工艺研究

Enriching Technic-process of Total Flavonoids from the Aerial-part of Sonchus Arvensis L.

以总黄酮回收率为指标,通过静态吸附和解吸附筛选最佳树脂,确立苦菜地上部分总黄酮的富集纯化工艺。通过静态吸附与解吸附对9种不同极性的大孔树脂进行筛选,优化最佳树脂的上样pH值、上样浓度、上样流速、洗脱流速、洗脱浓度、洗脱体积和径高比等工艺参数。AB-8树脂是富集纯化苦菜地上部分总黄酮的最佳树脂,最佳工艺参数为:上样pH值为pH=5.0,上样液总黄酮质量浓度为0.672 3 mg/mL,吸附流速为2 mL/min,解吸附流速为3 mL/min,乙醇洗脱液的浓度为70%,洗脱体积为2 BV,树脂柱径高比为1∶10,大孔树脂最多重复使用3次,总黄酮纯度倍数为2.43。AB-8型大孔树脂能有效地富集纯化苦菜地上部分总黄酮。

Filter the best macroporous resin and establish purification technology of total flavonoids from the aerial part of Sonchus arvensis L. with the recovery index of total flavonoids as the reference. The best macroporous resin was filtered among the nine resins by investigating the static adsorption and desorption properties through experiments, and then opti- mize the separation process of total flavonoids from the aerial part of Sonchus arvensis L. by using the best resin. The re- sults showed that the AB -8 macroporous resin had good adsorption and desorption. The optimal technological conditions were ： pH = 5.0, initial concentration 0.672 3 mg/mL, flow rate of adsorption 2 mL/min, 2BV , 70% ethanol, and de- sorption flow rate 3 mL/min. The diameter height ratio of resin column was 1：10 and the macroporous resin could be used under 3 times. Under this condition, the purity of total flavonoids had been increased from 1.61% to 3.91%. The AB - 8 maeroporous resin could be used to purify the total flavonoids from the aerial part of Sonchus arvensis L.