紫外-可见分光光度法定量测定姜黄素及其载体药物方法的优化

Optimization of quantitative method for curcumin and its carrier drugs by ultraviolet-visible spectrophotometry

目的利用紫外-可见分光光度法建立一种简便快速的姜黄素及载体中药物含量的测定方法。方法采用碱液法测定载体药物中包载的姜黄素的含量,将姜黄素载体药物溶于10 g/L氢氧化钠溶液中,分离出载体后在最大吸收波长470 nm处用紫外-可见分光光度计对姜黄素含量进行测定,根据标准曲线计算出载药量。结果姜黄素在8.0~15.0 mg/L浓度(x)下与吸光度(y)有很好的线性关系,得到回归方程为y=0.139x+0.009,r^2=0.999(n=7),1 h内误差<0.4 mg/L。结论该法方便快捷,廉价环保,可用于姜黄素及载体包载量的定量研究。

Objective To set up a convenient and rapid quantitative method for eureumin and its carrier drugs by ultravioletvisible spectrophotometry. Methods Alkali-soluble method was used to determine the content of curcumin in its drug carriers. A certain amount of drug carriers containing cureumin was dissolved in 10 g/L sodium hydroxide solution and measured at maximum adsorption wavelength of 470 ran. The encapsulation ratio of drug carriers was determined according to the result of measurement using a calibration curve as a reference. Results The concentration （x） and absorbance （y） had a good linear correlation in the range of 8.0- 15.0 mg/L. The regression equation was as follows： y=0.139x＋0.009, r^2=0.999 （n=7）. The deviation was less than 0.4 mg/L within an hour. Conclusion This method is simple, rapid and accurate, with no pollution to the environment, which can be used for the entrapment efficiency and quality standards of curcumin nanoparticles.