期刊文献+

人原代角质形成细胞在两种培养基中增殖能力的比较

Comparisons of the cell proliferation capacity of human primary keratinocytes in two different media
下载PDF
导出
摘要 目的:比较第一代和第二代人原代角质形成细胞(human primary keratinocytes,HPK)在两种培养基[(EpiLife培养基+HKGS)和DK-SFM培养基]中增殖能力的差异。方法:通过两步法从人的皮肤组织获得第一代HPK,进行传代后获得第二代HPK,采用细胞计数仪计数,绘制生长曲线。用免疫荧光技术鉴定第二代HPK,并用流式细胞术检测其细胞周期。结果:从HPK生长曲线可见,在DK-SFM培养基中,第一代HPK的增殖速率高于(EpiLife培养基+HKGS)中,第二代HPK的增殖速率低于(EpiLife培养基+HKGS)培养中。细胞周期结果同上,第二代HPK在DK-SFM中其G2期细胞和S期细胞含量分别为8.5%和11.2%,低于在(EpiLife培养基+HKGS)中的13.9%和18.3%。结论:两种培养基培养HPK各有利弊,若想在短期获得纯度较高的HPK,选择DK-SFM培养基较好;若想相对长期培养HPK,选择(EpiLife培养基+HKGS)更有优势。 Objective, To compare the differences in proliferation abilities of the first and second generations of human pri- mary keratinocytes (HPK) in two different media [EpiLife medium + HKGS and defined-keratinocyte serum-free medium(DK- SFM)]. Methods. The first generation of HPK was isolated from the skin by two-step digestion technique. The second genera- tion of HPK was obtained after passage, Cell growth curves were made by cell counter. The second generation of HPK were identified by immunofluorescence staining, and the cell cycle was determined by flow cytometry. Results: In the growth curves of HPK, the cell proliferation rate of the first generation of HPK was superior in DK-SFM than that in (EpiLife medium + HKGS). However, the proliferation rate of second generation of HPK in DK-SFM was lower than that in (EpiLife medium + HKGS) . The cell cycle experiment showed that after passage, the G2 phase and S phase cell contents were 8.5% and 11.2% in DK SFM, which were lower than those in (EpiLife medium + HKGS)(G2:13.9%, S:18.3 0% ). Conclusion: Both media have their own advantages and disadvantages. If relatively pure HPK is expected in a short time, it is better to select the DK-SFM medium. On the other hand, if prolonged culture of HPK is desired, it is more advantageous to choose (EpiLife Medium+ HKGS).
出处 《药学服务与研究》 CAS 2017年第2期114-119,共6页 Pharmaceutical Care and Research
关键词 角质形成细胞 细胞培养技术 EpiLife培养基 defined-keratinocyte serum-free培养基 keratinocyte cell culture technique EpiLife medium defined-keratinocyte serum free medium
  • 相关文献

参考文献5

二级参考文献74

  • 1姜薇,孙莹,赵俊郁,孙笑,史春艳,卜定方,朱学骏.Hallopeau-Siemens型营养不良型大疱性表皮松解症一例产前诊断[J].中华皮肤科杂志,2006,39(2):80-82. 被引量:6
  • 2Shao-Chih Chiu,Huey-Shan Hung,Shinn-Zong Lin,Esheral Chiang,Demeral David Liu.Therapeutic potential of olfactory ensheathing cells in neurodegenerative diseases[J].Journal of Molecular Medicine.2009(12)
  • 3J. Y. K. Leung,J. A. Chapman,J. A. Harris,D. Hale,R. S. Chung,A. K. West,M. I. Chuah.Olfactory ensheathing cells are attracted to, and can endocytose, bacteria[J].Cellular and Molecular Life Sciences.2008(17)
  • 4Lu ZF,Shen YX,Zhang P,et al.Ginsenoside Rg1 promotes proliferation and neurotrophin expression of olfactory ensheathing cells[].Journal of Asian Natural Products Research.2010
  • 5Zhu Y,,Cao L,Su Z,et al.Olfactory ensheathing cells:attractant of neural progenitor migration to olfactory bulb[].Glia.2010
  • 6Pellitteri R,Spatuzza M,Russo A,et al.Olfactory ensheathing cells represent an optimal substrate for hippocampal neurons:an in vitro study[].International Journal of Developmental Neuroscience.2009
  • 7Shukla S,Chaturvedi RK,Seth K,et al.Enhanced survival and function of neural stem cells-derived dopaminergic neurons under influence of olfactory ensheathing cells in parkinsonian rats[].Journal of Neurochemistry.2009
  • 8Runyan SA,Phelps PE.Mouse olfactory ensheathing glia enhance axon outgrowth on a myelin substrate in vitro[].Experimental Neurology.2009
  • 9Radtke C,Sasaki M,Lankford KL,et al.Potential of olfactory ensheathing cells for cell-based therapy in spinal cord injury[].Journal of Rehabilitation Research and Development.2008
  • 10Fouad K,Pearse DD,Tetzlaff W,et al.Transplantation and repair:combined cell implantation and chondroitinase delivery prevents deterioration of bladder function in rats with complete spinal cord injury[].Spinal Cord.2009

共引文献10

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部