Fox O1在结直肠腺瘤及腺癌组织中的表达及其与凋亡的相关性研究

Expression of Fox O1 Protein in Colorectal Adenoma and Adenocarcinoma and Research on its Correlation with Apoptosis

为探讨FoxO1在结直肠腺瘤及腺癌组织中的表达及其与组织凋亡率、凋亡因子表达的相关性，本研究采用免疫组化和Westernblot法检测146例结直肠腺瘤、48例结直肠腺癌及癌旁正常组织中FoxO1蛋白的表达，采用Westernblot和Tunnel法检测凋亡因子（Fas、Bim和Caspase-3）的表达及组织凋亡率，并分析两者与FoxO1蛋白表达的相关性。结果显示，结直肠腺癌组和高级别瘤变组FoxO1阳性率明显低于低级别瘤变组和癌旁正常黏膜组，P〈0．05或P〈0．01。Fas、Bim和Caspase-3蛋白的表达在“正常结直肠黏膜一癌”的续变过程中也逐渐降低，且三者的表达水平均与FoxO1的表达相关，P〈0．01。Tunnel检测显示，各组组织凋亡率比较差异均有统计学意义，P〈0．01；但此差异与FoxO1的差异表达无相关性，P〉0．05。结果表明，FoxO1在结直肠腺瘤及腺癌组织细胞核表达的减少和缺失可能是导致结直肠腺癌发病的机制之一。FoxO1参与调控结直肠癌变过程中凋亡途径上关键因子的表达，但其在癌变的续变过程中的调控功能可能不是主要通过调控凋亡过程来实现的。

To explore the expression of Fox O1 in colorectal adenoma and adenocarcinoma and its correla- tion with the tissue apoptosis rate and the expression of apoptosis factor, the expression of Fox O1 protein in 146 cases of colorectal adenoma,48 eases of eolorectal adenocacinoma and para-carcinoma normal tissues were detected by immunohistochemistry and Western blot. The expression of apoptosis factors （Fas, Bim and Caspase 3） and tissue apoptosis rate were detected by the method of Western blot and Tunnel,and the correlation of the two aspects and the expression of Fox O1 protein was analyzed. As results,the positive rate of Fox O1 in eoloreetal adenocarcinoma group and high-grade neoplasia group was significantly lower than that in low-grade neoplasia group and para-earcinoma normal mucosa ＇group （ P ~0.05 or P （0.01）. The protein expressions of Fas, Bim and Caspase-3 were also decreased inthe eontinous change course from ＂normal colorectal mucosa to cancer＂. The expression levels of Fas,Bim and Caspase-3 were all correlated with the expression of Fox O1 （P〈0.01）.The detection of Tunnel showed that there were significant differences in the apoptosis rate of each group （ P 〈0.01） ; but the differences were not correlated with the difference of Fox O1 expression, P 〈0.05.The results show that the descreased or lack expression of Fox O1 in coloreetal adenoma and adenocarcinoma cell core of tissue may be one of the mechanisms leading to colorectal adenocarcinoma. Fox O1 is involved in the regulation of the expression of key factors in the process of apoptosis of eoloreetal carcinogenesis, but its regulation in the proeess of cancerous continous changes may not be achieved mainly through the regulation of apoptosis process.