电磁噪声阻断极低频磁场对细胞缝隙连接功能的抑制效应

Electromagnetic noise blocks the gap-junctional intercellular communication suppression induced by 50 Hz magnetic field

目的 探讨电磁噪声对 5 0Hz磁场诱导的细胞缝隙连接通讯功能 (GJIC)抑制的干预作用。方法 小鼠成纤维细胞 (NIH 3T3)受 5 0Hz 0 .4mT极低频磁场或磁场加同等强度的电磁噪声联合作用 2 4h后 ,在激光共聚焦显微镜下 ,采用荧光光漂白后恢复技术 (FRAP)测定细胞的GJIC功能。结果 0 .4mT磁场单独作用明显抑制细胞的GJIC ,其荧光恢复率为 2 7.6 7%± 5 .12 % ,与对照组(45 .5 7%± 9.72 % )相比 ,差异有显著性 (P <0 .0 1) ;而磁场与电磁噪声联合作用 (5 2 .6 1%± 8.30 % )明显拮抗磁场对GJIC的抑制作用 ,与 0 .4mT磁场组的差异有显著性 (P <0 .0 1) ,并与对照组的差异无显著性 (P >0 .0 5 )。

Objective To explore whether the superposition of an electromagnetic noise can block gap junctional intercellular communication(GJIC) suppression induced by 50 Hz 0.4 mT extremely low frequency magnetic field(ELF MF). Methods Fibroblast cells of mice(NIH 3T3) were exposed to 0.4 mT ELF MF or(and) electromagnetic noise with the same intensity of MF for 24 h,and the GJIC was determined by using fluorescence recovery after photobleaching(FRAP) analysis,which was performed with a laser scanning confocal microscope(Leica,Germany). Results ELF MF exposure significantly inhibited GJIC with fluorescence recovery rate of 27.67%± 5.12% as compared with the control group(45.57%±9.72%)( P <0.01),while that of ELF MF plus noise group was (52.61%±8.30%),which was significantly different from ELF MF group(P<0.01),but not from control( P >0.05). Conclusion Electromagnetic noise could block the GJIC suppression induced by 50 Hz 0.4 mT MF.