电磁脉冲对大鼠肝脏线粒体膜流动性及脂质过氧化的影响

The effects of electromagnetic pulse on fluidity and lipid peroxidation of mitochondrial membrane

目的 研究高场强电磁脉冲对线粒体膜的生物学效应。方法 大鼠肝脏线粒体悬液经6 0kV/m电磁脉冲作用 ,运用电子顺磁共振 (ESR)波谱技术检测线粒体膜蛋白及膜脂运动性的变化 ,并采用分光光度法检测膜脂质过氧化的变化。结果 膜蛋白运动性由旋转相关时间 (τc)表示 ,脉冲作用后为 (0 .5 94± 0 .0 4 9)× 10 -9s,对照组为 (0 .5 0 1± 0 .0 77)× 10 -9s,差异有显著性 (P <0 .0 5 ) ,膜蛋白运动变慢 ;线粒体膜脂的有序性由序参数 (S)表示 ,脉冲作用后为 0 .6 1± 0 .0 1,对照组为 0 .6 3± 0 .0 1,差异有显著性 (P <0 .0 5 ) ,膜脂的有序性降低 ,运动加快 ;脂质过氧化产物丙二醛 (MDA)的含量脉冲作用后有所降低。结论 电磁脉冲作用使线粒体膜运动紊乱 。

Objective To study the effects of intense electromagnetic pulse(EMP) on the biological effects of mitochondrial membrane. Method Rat liver mitochondrial suspension was exposed to EMP at 60 kV/m level.The changes of membrane lipid fluidity and membrane protein mobility were detected by ESR and spin label technique.Malondialdehyde(MDA) was detected by spectrophotometer. Results The mobility of membrane protein decreased significantly( P <0.05).Correlation time( τ c ) of control group was (0.501±0.077)×10 -9 s,and τ c of EMP group was (0.594±0.049)×10 -9 s,indicating that the mobility of protein was restricted.The fluidity of mitochondrial membrane increased significantly( P <0.05) at the same time.Order parameter( S ) of mitochondrial membrane lipid in control group was 0.63±0.01,while S of EMP group was 0.61±0.01(P<0.05).MDA decreased significantly. Conclusion The mobility and lipid peroxidation of mitochondrial membrane may be disturbed after EMP exposure.