双酶法催化玉米淀粉制备γ-CD

γ-CD Catalyzed from Maize Starch by Dual Enzymatic Reaction

以玉米淀粉为底物,研究了来自于栖热水生菌的4α-糖基转移酶(4αGTase)和来自于嗜碱芽孢杆菌的环糊精葡萄糖基转移酶(CGTase)作用于淀粉制备γ-CD的影响因素。结果表明:两种酶的添加方式为先加入4αGTase、再加入CGTase;γ-CD的最佳制备条件为底物质量分数5%,4αGTase加酶量4 U/g淀粉,CGTase加酶量8U/g淀粉,反应时间30 h。在此条件下γ-CD的得率最高为12.83%,比对照组提高了76.7%。

Theconjunction of 4α-glycosyltransferase （4aGTase） with cyclodextrin glycosyltransferase （CGTase） were screened for their ability o^producing γ-cyclodextrin （CD） usingmaize starch as substrate. The studies on the influence factors showed that 4aGTase should be added first,followed byCGTase. The optimal conditions for γ-CD production was as following ：4U of 4αGTase and 8U ofCGTase per gram of substratewere used to convert maize starch（5%, w/ y） into 7-CD with the reaction time of 30 h. the subsequent yield of γ-CD was 12.83%, which was 76.7% higher than the control. This study has potential value for the industrial production of γ -CD.