脱细胞猪心包膜在引导骨组织再生术中的屏障作用

Experimental study on osteogenesis of acellular porcine pericardium in guided bone regeneration

目的 :在兔股骨骨缺损处行引导骨组织再生术,观察脱细胞猪心包膜的存留时间及引导骨再生的效果。方法 :自行制取脱细胞猪心包膜(acellular porcine pericardium,APP)。利用高、低渗Na Cl溶液将新鲜的猪心包交替浸泡后,胰酶+EDTA消化,Triton X-100漂洗,最后以戊二醛交联处理。选取24只新西兰大白兔,在双侧股骨远端设置8 mm×8 mm×5 mm骨缺损区,采用随机方法 ,一侧骨缺损区覆盖脱细胞猪心包膜(实验组),另一骨缺损区不盖膜(对照组)。分别于术后4、8和12周进行整体观察、X线片检查、组织学检查和骨密度测量。采用SPSS18.0软件包对数据进行统计学分析。结果:术后创口愈合良好,未发生感染等相关并发症;整体观察有纤维包囊覆盖脱细胞猪心包膜表面;实验组骨密度值较对照组显著升高(P<0.05);组织学观察见实验组成骨效果好于对照组。结论:利用脱细胞猪心包膜行引导骨组织再生术,可达到较理想的屏障作用,膜存留时间满足成骨需求。

PURPOSE： To observe the retention time and effect of acellular porcine pericardium（APP） in bone regeneration for rabbit femoral bone defects. METHODS： APP was prepared as follows： fresh porcine pericardium was chosen, high and low osmotic Na Cl solution were used to soak the specimen alternately, trypsin ＋EDTA were used for digest, and then immersed with Triton X-100, cross-linked by using glutaraldehyde. 24 New Zealand rabbits were selected,and the area of bone defect was created 8 mm×8 mm×5 mm in size on bilateral distal femur. Stochastic method was used for grouping. One side of bone defect was covered with APP（experimental group）; and the other side was covered without APP（control group）. General observation, X-ray examination, histological examination, and bone mineral density（BMD）were performed 4 weeks, 8 weeks and 12 weeks after operation. SPSS18.0 software package was used for statistical analysis. RESULTS： After surgery, all wounds healed without complications. Under general observation, there were some fibrous cysts covered on the surface of APP. BMD（P〈0.05） in the experimental group was significantly greater than that in the control group. On histological examination, the rate of osteogenesis in the experimental group was faster than that in the control group. CONCLUSIONS： Bone regeneration can be achieved using APP in the repair of rabbit bone defects.The retention time of APP can meet the needs of osteogenesis.