人牙源性iPSCs 3种培养底物的比较

Comparison among 3 kinds of culture substrates of odontogenic induced pluripotent stem cells

目的对比研究3种人牙源性诱导性多能干细胞(iPSCs)培养底物的特点。方法使用3种底物培养人牙源性iPSCs:小鼠胚胎成纤维细胞(MEF)、基质胶和重组人玻连蛋白(VTN-N),对比iPSCs的生长情况。结果 3种底物的制备时间分别为14、3、1h,三者间差异有统计学意义(P<0.05);iPSCs重编程时间分别为(30±1.6)、(26±2.1)、(27±1.4)d,其中MEF组明显多于其他两组(P<0.05);重编程效率分别为0.3%±0.03%、0.56%±0.08%、0.7%±0.02%(P<0.05)。3种底物均能较好支持iPSCs生长,使其保持未分化状态。结论重组人玻连蛋白无异源性动物组分,制备简便、标准可控、重编程时间较短,是目前理想的支持iPSCs生长的底物。

Objective To comparatively study the characteristics of 3 kinds of culture substrates of human odontogenic in- duced pluripotent stem cells（iPSCs）. Methods The human odontogenic iPSCs were cultured by 3 kinds of substrateszmouse em- bryonic fibroblasts （MEF）, matrigel and recombinant human vitronectin（VTN-N）. The iPSCs growth situation was compared a- mong three groups. Results The preparation time of these 3 kinds of substrates was 14,3,1 hlespectively, and, the difference was statistically significant （P〈0. 05）. The iPSCs reprogramming time was （30±1.6）, （26 ±2.1 ）, （27±1.4） d, lespectively, wht that in the MEF group significantly higer than in other two groups （P〈0.05）. The reprogramming efficiencies were 0. 3%± 0.03 %, 0.56%±0.08% ,0. 7 % ± 0.02 % respectively（P〈0.05）. Three kinds of substrate could better support iPSCs growth and make them to maintain un- differentiation status. Conclusion with no heterologous animal components, and the adrantaga of simple pteparation, oonfrollable standard and shorter gramming time is easy to prepare, the standard is controllable and the reprogramming time is shorter,which is an ideal substrate for supporting iPSCs growth.