间歇有氧运动上调自发性高血压大鼠血管肾上腺髓质素

Intermittent aerobic exercise up-regulated expression of adrenomedullin of spontaneous hypertensive rat's artery

目的研究间歇有氧运动(IAE)对自发性高血压大鼠(SHR)主动脉肾上腺髓质素(ADM)的影响,探讨运动降低血压的大血管因素及其机制。方法 SHR 30只,随机分为间歇有氧运动组(IAE组)和对照组。IAE组进行8周间歇跑台有氧运动。采用尾压法测血压,酶联免疫吸附法(ELISA)测运动前后血清中ADM的含量,免疫组织化学法显示SHR主动脉ADM的表达,蛋白质印迹实验检测主动脉ADM的特异性受体——受体活性修饰蛋白2(RAMP2)和降钙素受体样受体(CRLR)定量水平。结果 IAE组血压明显低于对照组(P<0.05);IAE组血清中ADM的含量明显高于对照组(P<0.05);主动脉ADM表达明显强于对照组(P<0.05);主动脉RAMP2和CRLR定量均明显高于对照组(P<0.05)。结论间歇有氧运动可能通过增强血管ADM及其特异性受体表达使SHR大鼠血压下降。

Aim To probe the effect of Intermittent aerobic exercise on Adrenomedullin （ADM） secretion of artery of spontaneous hypertensive rat （SHR） , and investigate the mechanism of artery of exercise in the prevention and treatment of hypertension. Methods 30 SHR are separated into two groups ： intermittent exercise group （ IAE Group） and control group. The IAE group performs 8 weeks intermittent treadmill exercise and their blood pressure are measured before and after exercise by tail pressure of SHR, serum ADM is detected by enzyme linked immunosorbent assay. ADM is expressed in artery by immunohistochemistry. Quantification of specific-receptor activity modifying protein （RAMP2） and calcitonin receptor like receptor（CRLR） in artery ADM are detected by Westernblotting. Results Quantification of specific receptor ADM were significantly lower than control group（P〈0.05） and serum ADM in IAE group were significandy higher than control group（P〈0.05）. The expression of ADM in artery was obviously stronger than control group. The quantity of RAMP2 and CRLR in artery were increased obviously （P〈0.05）. Condusion Intermittent aerobic exercise increases the expression of ADM in artery and their specific receptor RAMP2 and CRLR up regulation may be one of the mechanisms of lower SHR blood pressure.