绞缢对苹果矮砧压条新梢激素含量及生根相关基因表达的影响

Effect of Constriction on Content of Endogenous Hormones and Expression Rooting Related Genes During Shoot Layering of Apple Dwarfing Rootstock ‘9-3’

以1年生苹果矮化砧木‘9-3’为试材,研究了绞缢处理对压条新梢生根和生根过程中内源激素含量及生根相关基因表达的影响。结果表明:绞缢处理的新梢生根时间早于对照,且其生根率显著高于对照;在愈伤组织形成期,绞缢处理的新梢内IAA、ABA、ZR含量和IAA/ABA、IAA/GA_3、IAA/ZR比值均显著高于对照,ARRO-1、ARF7和ARF19基因表达量也显著高于对照;在不定根发生始期,绞缢处理的新梢内IAA和GA_3含量及ARRO-1基因的表达量均显著高于对照;在不定根发生盛期,绞缢处理的新梢内IAA含量和IAA/ABA、IAA/GA_3、IAA/ZR比值均显著低于对照,而ZR含量和ARRO-1基因表达量均显著高于对照。

One-year-old apple dwarfing rootstock‘9-3’was used as material to study the effect of constriction on rooting of layering shoots and the content of endogenous hormones and the expression of rooting related genes during the rooting process. The results showed that the rooting time of shoots treated with constriction was earlier than that of the control,and the rooting rate reached 90.63%,significantly higher than that of the control. At callus formation stage,the content of IAA,ABA and ZR,the ratio of IAA/ABA,IAA/GA3 and IAA/ZR in the treated shoots were significantly higher than those of the control. Meanwhile the expression of ARRO-1,ARF7 and ARF19 in the treated shoots was also significantly higher than that of the control. During the adventitious-root-formation stage,the content of IAA and GA3 and the expression of ARRO-1 in the treated shoots were significantly higher than those of the control. At the adventitious-root-formation peak stage,the content of IAA and the ratio of IAA/ABA,IAA/GA3 and IAA/ZR were significantly lower than those of the control,while the content of ZR and the expression of ARRO-1 were significantly higher than those of the control.