补肾活血化痰法对自发性高血压大鼠左心室纤维化的影响

Effect of Kidney-reinforcing, Blood-activating and Phlegm-resolving Therapy on Left Ventricular Fibrosis of Spontaneously Hypertensive Rats

【目的】探讨补肾活血化痰法改善自发性高血压大鼠(SHR)左心室纤维化机制。【方法】选用20只自发性高血压大鼠,随机分为模型组和中药组,10只WKY大鼠(Wistar-Kyoto rat)作为正常对照组。干预12周后,应用Masson染色法检测大鼠左心室纤维化程度,逆转录—聚合酶链反应(RT-PCR)法检测大鼠左心室Smad3 m RNA表达水平,Western blot法检测大鼠左心室Smad3表达水平。【结果】中药组大鼠心肌纤维化水平、Smad3蛋白表达水平、Smad3基因表达水平显著低于模型组(均P<0.05)。【结论】补肾活血化痰法可能通过抑制Smad3表达改善自发性高血压大鼠左心室纤维化进程。

Objective To explore the therapeutic mechanism of kidney-reinforcing, blood-activating and phlegm- resolving therapy for left ventricular fibrosis of spontaneously hypertensive rats （SHR）. Methods Twenty SHR were randomly assigned to Chinese medicine group and model group. Additionally, ten Wistar-Kyoto（WKY） rats served as normal control group. After 12-week prevention, Masson staining method was used to measure the degree of fibrosis of left ventricular myocardial tissues, reverse transcription-polymerase chain reaction（RT-PCR） was used to detect Smad3 mRNA expression, and Western blotting method was used for the detection of Smad3 protein expression. Results The degree of left ventricular fibrosis myocardial tissue in Chinese medicine group was milder than that in the model group, lower than those in the model resolving therapy can improve and Samd3 protein and mRNA expression levels in Chinese medicine group were group （P 〈 0.05）. Conclusion Kidney-reinforcing, blood-activating and phlegm- left ventricular fibrosis in SHR by inhibiting Smad3 expression.