中药材人参鉴定技术方法的研究与评价

Method Research and Evaluation of Identification Techniques on Traditional Chinese Medicine Ginseng

主要探讨现有的人参鉴定传统方法和分子生物学方法在鉴定过程中的准确性和客观性,综述了聚合酶链式反应(Polymerase Chain Reaction,PCR)、随机扩增多态性DNA标记(Randomly Amplified Polymorphic DNA,RAPD)、限制性片段长度多态性分析(Restriction Fragment Length Polymorphism,RFLP)、扩增片段长度多态性分析(Amplified Fragment Length Polymorphism,AFLP)、多位点探针DNA指纹图谱和微卫星标记技术(SSR)等在人参物种鉴定中的应用,并对每种技术进行了相应的评价.

The aim of this paper is to evaluate the identification of ginseng with traditional methods and molecular biological methods, and discusses the accuracy and objectivity of existing technology in the process of identification. The identification methods of Panax ginseng are applied such as： Polymerase Chain Reaction （PCR）, Randomly Amplified Polymorphic DNA （RAPD）, Restriction Fragment Length Polymorphism（ RFLP ）, Amplified Fragment Length Polymorphism （AFLP）, multilocus proke DNA fingerprint and SSR, each of identification methods is evaluated.