ASCL-1在肺腺癌组织中的表达及其临床意义

Clinical value of ASCL-1 protein expression in lung adenocarcinoma

目的检测ASCL-1基因在肺腺癌组织中的表达情况,分析其与肺神经内分泌标志物表达的相关性,研究ASCL-1成为临床病理诊断肺腺癌伴神经内分泌分化肿瘤标志物的可能性。方法应用免疫组化方法检测283例肺腺癌组织中ASCL-1、神经内分泌标志物(CgA、CD56、Syn)的表达并进行对比分析;同时采用Western blot对其中的63例肺腺癌新鲜组织标本中的ASCL-1蛋白表达进行检测。结果免疫组化显示,ASCL-1在肺腺癌组织中的表达阳性率为16.9%(48/283),CgA、Syn、CD56表达阳性率分别为4.2%(12/283)、22.2%(63/283)、5.3%(15/283)。在肺腺癌组织中ASCL-1的表达与神经内分泌标志物(CgA、Syn、CD56)的表达呈正相关性(0<r<1,P<0.05)。Western blot检测发现9例肺腺癌组织中有ASCL-1蛋白表达,而对应的癌旁正常肺组织中无ASCL-1蛋白表达。结论 ASCL-1基因对肺腺癌伴神经内分泌肿瘤具有相关性,联合检测ASCL-1与CgA、Syn、CD56可提高诊断率。

Objective This study is to detect ASCL-1 expression in lung adenocarcinoma（AD）, to analyze its expression correlated with traditional neuroendocrine （NE） markers, and to research the possibility of ASCL-1 as clinical pathological markers in AD with NE differentiation. Methods ASCL-1 protein expression by immunohistochemical analysis in 283 cases of AD and compared with expression of traditional NE markers （ Chromogranin A,CD56 ,Synaptophysin）. Western blot was applied to detect the expressions of ASCL-1 protein in 63 cases of AD. Results By immunohistochemical, ASCL-1 expression was positive in 48/283 （ 16. 9% ） AD, the positive rate of CgA was 4. 2% （ 12/283）, the positive rate of Syn was （63/283）, and the positive rate of CD56 was 5.3% （ 15/283 ）. , and positive correlation with NE markers （0 〈 r 〈 1 ,P 〈 0.05 ）. Western blot test in 63 cases of AD and tumor-adjacent normal samples, 9 eases of AD express ASCL-1 protein, and tumor-adjacent normal tissue without protein expression. Conclusion ASCL-1 is a new kind of correlative marker which may be applied to clinical pathology diagnosis of AD with NE differentiation, Joint detection with CgA 、Syn、CD56 may improve the diagnostic rate.