姜黄素类似物对Aβ_(25-35)诱导的AD细胞模型细胞凋亡的影响

Effects of curcumin analogues on cell apoptosis induced by Aβ25-35 in AD cell model

目的研究姜黄素类似物H8对Aβ_(25-35)(β-amyloid protein 25-35)诱导的PC12细胞凋亡相关基因Caspase-3、Bcl-2、Bax表达的影响。方法 CCK-8法检测Aβ_(25-35)及姜黄素类似物H8的细胞毒性,以Aβ_(25-35)诱导PC12细胞(大鼠肾上腺嗜铬细胞瘤细胞)建立AD细胞模型,并使用不同浓度的H8进行干预,通过RT-q PCR法检测各组凋亡相关基因Caspase-3、Bcl-2、Bax mRNA表达。结果 H8在实验浓度范围内几乎无细胞毒性,Aβ_(25-35)具有明显的细胞毒性并呈浓度依赖性,经过H8干预后细胞存活率明显升高,其中以20μmol/L的H8作用最明显(P<0.001)。经过H8作用后能降低Aβ_(25-35)诱导的Caspase-3、Bax mRNA表达,升高Bcl-2 mRNA表达。结论姜黄素类似物H8能够抑制Aβ_(25-35)诱导的凋亡相关基因,发挥抗凋亡作用。

Objective To investigate the effect of curcumin analogue H8 on the expressionof apoptosis-related genes Caspase-3, Bcl-2 and Bax in PC12 cells induced by Aβ25-35 （β-amyloid protein 25-35）. Methods The cytotoxicity of Aβ25-35 and curcumin analogue H8 were detected by CCK-8. The AD cell model was established using PC12 cells （rat adrenal pheochromocytoma cells） induced by Aβ25-35 and the ceils were treated with different concentrations of H8. The expression of Caspase-3, Bcl-2 and Bax mRNA were detected by RT-qPCR. Results H8 had almost no cytotoxicity in the experimental concentration range, while Aβ25-35 had obvious cytotoxicity and concentration-dependent. The cell sur- vival rate was significantly increased after H8 intervention, among them, 20 μmol/L H8 was most significantly（P〈0.001）. After treatment with H8 could reduce Aβ25-35-induced Caspase-3, Bax mRNA expression and increase Bcl-2 mRNA expression. Conclusion Curcumin analogues H8 can inhibit Aβ25-35-induced apoptosis-related genes, with anti-apoptotic effect：