摘要
采用水提醇沉法提取裂褶菌多糖,用磺酰化法对其进行硫酸酯化,经红外光谱定性分析,硫酸钡比浊法定量计算得出硫酸基的含量。采用邻二氮菲-Fe^(2+)氧化法测定裂褶菌多糖和裂褶菌硫酸酯对羟自由基的清除作用。结果表明:硫酸基含量为13.9%,硫酸基取代度(DS)为1.26时,硫酸酯化裂褶菌多糖的抗氧化活性比裂褶菌多糖增加2.6倍。硫酸酯化修饰能提高裂褶菌多糖的抗氧化活性。
The Schizophyllan polysaccharide was extracted by water extraction and ethanol precipitation method. The polysaccharide esterification were synthetized by chlorosulfonic acid method and was confirmed by infrared spectroscopy. The content of sulfate radical was determined by barium sulfate turbidity. The effects of polysaccharides scavenging hydroxyl were determined by phenanthroline-metal iron-H2O2 system. The results showed that：when sulfate replaced was 13.9 %,sulfation rate of 1.26 sulfated Schizophyllan polysaccharide antioxidant activity increased by 2.6 times than the unmodified polysaccharide. The antioxidant activity of Schizophyllan polysaccharide can be improved through sulfation.
作者
张虽栓
蔡花真
ZHANG Sui-shuan CAI Hua-zhen(Henan Vocational College of Quality Engineering, Pingdingshan 467000, Henan, Chin)
出处
《食品研究与开发》
CAS
北大核心
2017年第8期17-21,共5页
Food Research and Development
基金
河南省科技攻关项目(142102210222c)
关键词
裂褶菌多糖
硫酸酯化
硫酸基鉴定
抗氧化
Schizophyllan polysaccharide
sulfation
sulfuric acid-based identification
antioxidant
