不同途径注射卵蛋白致大鼠过敏性休克的研究

Development of anaphylactic shock rat models induced by ovalbumin injected via different routes

目的:建立不同途径致大鼠过敏性休克动物模型,找到相对高效的造模方式。方法:将健康SD大鼠随机分为正常对照组和致敏1、2、3组,致敏组分别于第1天不同部位注射致敏原,第5天加强注射1次,饲养第18天不同部位注射致敏原激发过敏性休克。结果:(1)致敏组大鼠均出现过敏性休克的典型体征,且尾静脉注射致敏原激发过敏性休克致敏组的症状比较严重;(2)致敏组与正常对照组比较血清Ig E、类糜蛋白酶含量升高,致敏1组血清含量高于其他致敏组,差异具有统计学意义(P<0.05)。结论:本实验成功地建立了大鼠过敏性休克动物模型,证明最后一次激发大鼠过敏性休克的注射途径,应选择尾静脉比较理想。

Objective：To verify a relatively efficient modeling technique for anaphylactic shock in rats induced by different routes of administration of ovalbumin.Methods：Healthy SD rats were randomly divided into normal control group and sensitized group 1,2 and 3.Rats in the three sensitized groups were sensitized by injection of the allergen via different routes in the day 1,and additional dose in the day 5.Then the rats were fed for 18 days,and challenged for the anaphylactic shock in the 18th day by ovalbumin administration in different routes.Results：①Rats in the sensitized group showed typical signs of anaphylactic shock in general,yet the symptoms appeared severer in rats sensitized by injection of the allergen via tail vein;②Rats in the sensitized group had higher levels of IgE and chymase than the control group,and higher serum IgE and chymase levels were observed in sensitized group 1（P〈0.05）.Conclusion：Anaphylactic shock model was successfully developed in rats.However,the trials indicate that injection of the allergen via tail vein will result in optimal modeling in such animals.