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实时荧光定量PCR检测人脑胶质瘤、髓母细胞瘤中SAMD 14的表达

Expression of SAMD14 in human glioma and medulloblastoma detected by quantitative real time PCR
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摘要 目的分析人胶质瘤和髓母细胞瘤中SAMD 14 mRNA的表达情况及其与临床参数间的关系。方法使用实时荧光定量PCR(qRT-PCR)检测10例正常人脑组织、24例胶质瘤患者和11例髓母细胞瘤患者肿瘤组织中SAMD 14 mRNA的相对表达量,分析其表达与患者临床病理学特征的关系。结果胶质瘤、髓母细胞瘤组织中SAMD 14 mRNA的表达水平低于正常脑组织,胶质瘤中SAMD14 mRNA的表达水平高于髓母细胞瘤组织(P<0.05)。SAMD 14 mRNA相对表达量与性别、年龄、肿瘤直径和KPS评分等因素无关(P>0.05)。结论 SAMD 14 mRNA的低表达可能与胶质瘤和髓母细胞瘤的发生、发展有关。 ObjectiveTo analyze the expression of SAMD 14 mRNA in human glioma and medulloblastoma ，and to study their association with clinical features of glioma and medulloblastoma. MethodsThe expression of SAMD 14 mRNA was detected in 10 human normal brain tissues, 24 glioma tissues and 11 medulloblastoma tissues by qRT-PCR, then the relationship of their expression and clinical features were analyzed. ResultsThe expression of SAMD 14 mRNA was lower in glioma and medulloblastoma tissues than in human normal brain tissues. The expression of SMAD14 mRNA was higher in glioma tissues than in medulloblastoma tissues（P＜0.05）. There is no significant difference between the expression of SMAD14 mRNA and clinical features（Sex, age, tumor diameter and KPS scores,etc）. ConclusionThe low expression of SAMD 14 were possibly related to tumorigenesis of glioma and medulloblastoma.
作者 龚范勇 葛盈盈 肖绍文
机构地区 广西医科大学第一附属医院 广西医科大学基础医学院广西高校基础医学重点实验室
出处 《微创医学》 2017年第2期162-165,186,共5页 Journal of Minimally Invasive Medicine
基金 国家自然科学基金(编号:81460382 81560408) 广西自然科学基金(编号:2014GXNSFAA118231 2014GXNSFAA118089) 广西医科大学青年科学基金(编号:GXMUYSF201409)
关键词 胶质瘤 髓母细胞瘤 SAMD 14 荧光定量PCR Glioma Medulloblastoma SAMD 14 qRT-PCR
分类号 R739.41 [医药卫生—肿瘤]
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微创医学
2017年 第2期

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